Name: Anti-EIF3G Antibody (CAB4240)
Brand: Metabolism Antibodies 2
SKU: CAB4240
Availability: OutOfStock

Antibody Name:	Anti-EIF3G Antibody
Antibody SKU:	CAB4240
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human EIF3G (NP_003746.2).

Application:	WB IF
Recommended Dilution:	WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SH-SY5Y, Jurkat, HeLa, NIH/3T3, HL-60, K-562, Mouse Kidney, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human EIF3G (NP_003746.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPTG DFDS KPSW ADQV EEEG EDDK CVTS ELLK GIPL ATGD TSPE PELL PGAP LPPP KEVI NGNI KTVT EYKI DEDG KKFK IVRT FRIE TRKA SKAV ARRK NWKK FGNS EFDP PGPN VATT TVSD DVSM TFIT SKED LNCQ EEED PMNK LKGQ KIVS CRIC KGDH WTTR CPYK DTLG PMQK ELAE QLGL STGE KEKL PGEL EPVQ ATQN KTGK YVPP SLRD GASR RGES MQPN RRAD DNAT IRVT NLSE DTRE TDLQ ELFR PFGS ISRI YLAK DKTT GQSK GFAF ISFH RRED AARA IAGV SGFG YDHL ILNV EWAK PSTN
Gene ID:	8666
Uniprot:	O75821
Cellular Location:	Cytoplasm, Nucleus, perinuclear region
Calculated MW:	35kDa
Observed MW:	42kDa

Synonyms:	EIF3G, EIF3-P42, EIF3S4, eIF3-delta, eIF3-p44
Background:

UniProt Protein Function:	eIF3-delta: eukaryotic translation initiation factor 3 subunit 4. Binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. This subunit binds to the 18S rRNA. eIF-3 is composed of at least 12 different subunits. Contains 1 RNA recognition motif (RRM) domain.
UniProt Protein Details:	Protein type:Translation initiation; Translation; RNA-binding Chromosomal Location of Human Ortholog: 19p13.2 Cellular Component: eukaryotic translation initiation factor 3 complex; perinuclear region of cytoplasm; cytoplasm; nucleus; cytosol Molecular Function:protein binding; translation initiation factor activity; nucleotide binding Biological Process: cellular protein metabolic process; translation; translational initiation; gene expression; formation of translation preinitiation complex; regulation of translational initiation
UniProt Code:	O75821
NCBI GenInfo Identifier:	23503064
NCBI Gene ID:	8666
NCBI Accession:	O75821.2
UniProt Secondary Accession:	O75821,O14801, Q969U5,
UniProt Related Accession:	O75821
Molecular Weight:	35,611 Da
NCBI Full Name:	Eukaryotic translation initiation factor 3 subunit G
NCBI Synonym Full Names:	eukaryotic translation initiation factor 3, subunit G
NCBI Official Symbol:	EIF3G
NCBI Official Synonym Symbols:	EIF3S4; EIF3-P42; eIF3-p44; eIF3-delta
NCBI Protein Information:	eukaryotic translation initiation factor 3 subunit G; eIF3 p42; eIF3 p44; eIF-3-delta; eIF-3 RNA-binding subunit; eukaryotic translation initiation factor 3 subunit 4; eukaryotic translation initiation factor 3 subunit p42; eukaryotic translation initiation factor 3 RNA-binding subunit; eukaryotic translation initiation factor 3, subunit 4 delta, 44kDa
UniProt Protein Name:	Eukaryotic translation initiation factor 3 subunit G
UniProt Synonym Protein Names:	Eukaryotic translation initiation factor 3 RNA-binding subunit; eIF-3 RNA-binding subunit; Eukaryotic translation initiation factor 3 subunit 4; eIF-3-delta; eIF3 p42; eIF3 p44
UniProt Gene Name:	EIF3G
UniProt Entry Name:	EIF3G_HUMAN

	Western blot analysis of extracts of various cell lines, using EIF3G antibody (CAB4240) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of U-2 OS cells using EIF3G Polyclonal Antibody (CAB4240) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.