Metabolism Antibodies 2
Anti-EIF3F Antibody (CAB7023)
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- SKU:
- CAB7023
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-EIF3F Antibody |
| Antibody SKU: | CAB7023 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 88-357 of human EIF3F (NP_003745.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HL-60, SKOV3, BT-474, Jurkat, HeLa, Mouse spleen, Mouse lung, Mouse heart, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 88-357 of human EIF3F (NP_003745.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GGRV VRLH PVIL ASIV DSYE RRNE GAAR VIGT LLGT VDKH SVEV TNCF SVPH NESE DEVA VDME FAKN MYEL HKKV SPNE LILG WYAT GHDI TEHS VLIH EYYS REAP NPIH LTVD TSLQ NGRM SIKA YVST LMGV PGRT MGVM FTPL TVKY AYYD TERI GVDL IMKT CFSP NRVI GLSS DLQQ VGGA SARI QDAL STVL QYAE DVLS GKVS ADNT VGRF LMSL VNQV PKIV PDDF ETML NSNI NDLL MVTY LANL TQSQ IALN EKLV NL |
| Gene ID: | 8665 |
| Uniprot: | O00303 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 37kDa |
| Observed MW: | 42kDa |
| Synonyms: | EIF3F, EIF3S5, eIF3-p47 |
| Background: |
| UniProt Protein Function: | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773). |
| UniProt Code: | O00303 |
| NCBI GenInfo Identifier: | 4503519 |
| NCBI Gene ID: | 8665 |
| NCBI Accession: | NP_003745.1 |
| UniProt Secondary Accession: | O00303,Q6IB45, Q8N978, A8K0S2, |
| UniProt Related Accession: | O00303 |
| Molecular Weight: | 38 KD |
| NCBI Full Name: | eukaryotic translation initiation factor 3 subunit F |
| NCBI Synonym Full Names: | eukaryotic translation initiation factor 3 subunit F |
| NCBI Official Symbol: | EIF3F |
| NCBI Official Synonym Symbols: | EIF3S5; eIF3-p47 |
| NCBI Protein Information: | eukaryotic translation initiation factor 3 subunit F |
| UniProt Protein Name: | Eukaryotic translation initiation factor 3 subunit F |
| UniProt Synonym Protein Names: | Deubiquitinating enzyme eIF3f (EC:3.4.19.12); Eukaryotic translation initiation factor 3 subunit 5UniRule annotationManual assertion according to rulesiHAMAP-Rule:MF_03005; eIF-3-epsilonUniRule annotationManual assertion according to rulesiHAMAP-Rule:MF_03005; eIF3 p47UniRule annotationManual assertion according to rulesiHAMAP-Rule:MF_03005 |
| UniProt Gene Name: | EIF3F |
 | Western blot analysis of extracts of various cell lines, using EIF3F antibody (CAB7023) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded rat brain using EIF3F Antibody (CAB7023) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using EIF3F Antibody (CAB7023) at dilution of 1:100 (40x lens). |
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