Name: Anti-EIF3C Antibody (CAB7022)
Brand: Metabolism Antibodies 2
SKU: CAB7022
Availability: OutOfStock

Antibody Name:	Anti-EIF3C Antibody
Antibody SKU:	CAB7022
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 694-913 of human EIF3C (NP_003743.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, HL-60, HepG2, SW620, THP-1, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 694-913 of human EIF3C (NP_003743.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EIPY MAAH ESDA RRRM ISKQ FHHQ LRVG ERQP LLGP PESM REHV VAAS KAMK MGDW KTCH SFII NEKM NGKV WDLF PEAD KVRT MLVR KIQE ESLR TYLF TYSS VYDS ISME TLSD MFEL DLPT VHSI ISKM IINE ELMA SLDQ PTQT VVMH RTEP TAQQ NLAL QLAE KLGS LVEN NERV FDHK QGTY GGYF RDQK DGYR KNEG YMRR GGYR QQQS QTAY
Gene ID:	8663
Uniprot:	Q99613
Cellular Location:	Cytoplasm
Calculated MW:	104kDa/105kDa
Observed MW:	105kDa

Synonyms:	EIF3C, EIF3CL, EIF3S8, eIF3-p110
Background:

UniProt Protein Function:	EIF3C: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Identified in a HCV IRES-mediated translation complex, at least composed of EIF3C, IGF2BP1, RPS3 and HCV RNA-replicon. Belongs to the eIF-3 subunit C family.
UniProt Protein Details:	Protein type:Translation; RNA-binding; Translation initiation Chromosomal Location of Human Ortholog: 16p11.2 Cellular Component: cytosol; eukaryotic translation initiation factor 3 complex Molecular Function:protein binding; translation initiation factor activity; translation initiation factor binding Biological Process: translational initiation
UniProt Code:	Q99613
NCBI GenInfo Identifier:	6685539
NCBI Gene ID:	8663
NCBI Accession:	Q99613.1
UniProt Secondary Accession:	Q99613,O00215, Q9BW98, A8K7Z0, B2RXG3, B4E1D5, H3BRV0
UniProt Related Accession:	Q99613
Molecular Weight:	104,101 Da
NCBI Full Name:	Eukaryotic translation initiation factor 3 subunit C
NCBI Synonym Full Names:	eukaryotic translation initiation factor 3 subunit C
NCBI Official Symbol:	EIF3C
NCBI Official Synonym Symbols:	EIF3CL; EIF3S8; eIF3-p110
NCBI Protein Information:	eukaryotic translation initiation factor 3 subunit C
UniProt Protein Name:	Eukaryotic translation initiation factor 3 subunit C
UniProt Synonym Protein Names:	Eukaryotic translation initiation factor 3 subunit 8
UniProt Gene Name:	EIF3C
UniProt Entry Name:	EIF3C_HUMAN

	Western blot analysis of extracts of various cell lines, using EIF3C antibody (CAB7022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat brain using EIF3C Antibody (CAB7022) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse intestine using EIF3C Antibody (CAB7022) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using EIF3C Antibody (CAB7022) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using EIF3C antibody (CAB7022). Blue: DAPI for nuclear staining.