Metabolism Antibodies 1

Anti-EIF3B Antibody (CAB10259)

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SKU:
CAB10259
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-EIF3B Antibody
Antibody SKU:CAB10259
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 100-350 of human EIF3B (NP_003742.2).
Application:WB IHC IF
Recommended Dilution:WB 1:1000 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, Jurkat, HT-1080, LO2, NIH/3T3, Rat thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 100-350 of human EIF3B (NP_003742.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PVPA QGEA PGEQ ARDE RSDS RAQA VSED AGGN EGRA AEAE PRAL ENGD ADEP SFSD PEDF VDDV SEEE LLGD VLKD RPQE ADGI DSVI VVDN VPQV GPDR LEKL KNVI HKIF SKFG KITN DFYP EEDG KTKG YIFL EYAS PAHA VDAV KNAD GYKL DKQH TFRV NLFT DFDK YMTI SDEW DIPE KQPF KDLG NLRY WLEE AECR DQYS VIFE SGDR TSIF WNDV KDPV SIEE RARW TETY VRWS PKGT YLA
Gene ID:8662
Uniprot:P55884
Cellular Location:Cytoplasm
Calculated MW:92kDa/99kDa
Observed MW:125kDa
Synonyms:EIF3B, EIF3-ETA, EIF3-P110, EIF3-P116, EIF3S9, PRT1
Background:
UniProt Protein Function:eIF3-eta: eukaryotic translation initiation factor 3 subunit 9. Binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. eIF-3 is composed of at least 12 different subunits.
UniProt Protein Details:

Protein type:Translation; Translation initiation

Chromosomal Location of Human Ortholog: 7p22.3

Cellular Component: cytosol; eukaryotic translation initiation factor 3 complex

Molecular Function:protein binding; protein complex scaffold; RNA binding; translation initiation factor activity

Biological Process: regulation of translational initiation; translational initiation

UniProt Code:P55884
NCBI GenInfo Identifier:218512094
NCBI Gene ID:8662
NCBI Accession:P55884.3
UniProt Secondary Accession:P55884,Q2NL77, Q9UMF9, A4D208,
UniProt Related Accession:P55884
Molecular Weight:92kDa
NCBI Full Name:Eukaryotic translation initiation factor 3 subunit B
NCBI Synonym Full Names:eukaryotic translation initiation factor 3 subunit B
NCBI Official Symbol:EIF3B  
NCBI Official Synonym Symbols:PRT1; EIF3S9; EIF3-ETA; EIF3-P110; EIF3-P116  
NCBI Protein Information:eukaryotic translation initiation factor 3 subunit B
UniProt Protein Name:Eukaryotic translation initiation factor 3 subunit B
UniProt Synonym Protein Names:Eukaryotic translation initiation factor 3 subunit 9
UniProt Gene Name:EIF3B  
Anti-EIF3B Antibody (CAB10259)Western blot analysis of extracts of various cell lines, using EIF3B antibody (CAB10259) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-EIF3B Antibody (CAB10259)Immunohistochemistry of paraffin-embedded human breast cancer using EIF3B antibody (CAB10259) at dilution of 1:100 (40x lens).
Anti-EIF3B Antibody (CAB10259)Immunohistochemistry of paraffin-embedded human lung cancer using EIF3B antibody (CAB10259) at dilution of 1:100 (40x lens).
Anti-EIF3B Antibody (CAB10259)Immunofluorescence analysis of HeLa cells using EIF3B Polyclonal Antibody (CAB10259) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-EIF3B Antibody (CAB10259)Immunofluorescence analysis of NIH-3T3 cells using EIF3B Polyclonal Antibody (CAB10259) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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