Metabolism Antibodies 2
Anti-EIF2S3 Antibody (CAB6581)
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- SKU:
- CAB6581
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-EIF2S3 Antibody |
| Antibody SKU: | CAB6581 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 173-472 of human EIF2S3 (NP_001406.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HL-60, SW620 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 173-472 of human EIF2S3 (NP_001406.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | HLAA IEIM KLKH ILIL QNKI DLVK ESQA KEQY EQIL AFVQ GTVA EGAP IIPI SAQL KYNI EVVC EYIV KKIP VPPR DFTS EPRL IVIR SFDV NKPG CEVD DLKG GVAG GSIL KGVL KVGQ EIEV RPGI VSKD SEGK LMCK PIFS KIVS LFAE HNDL QYAA PGGL IGVG TKID PTLC RADR MVGQ VLGA VGAL PEIF TELE ISYF LLRR LLGV RTEG DKKA AKVQ KLSK NEVL MVNI GSLS TGGR VSAV KADL GKIV LTNP VCTE VGEK IALS RRVE KHWR LIGW GQIR RGVT IKPT VDDD |
| Gene ID: | 1968 |
| Uniprot: | P41091 |
| Cellular Location: | |
| Calculated MW: | 51kDa |
| Observed MW: | 51kDa |
| Synonyms: | EIF2S3, EIF2, EIF2G, EIF2gamma, MRXSBRK, eIF-2gA |
| Background: | The protein encoded by this gene is the largest subunit of a heterotrimeric GTP-binding protein involved in the recruitment of methionyl-tRNA(i) to the 40 S ribosomal subunit. |
| UniProt Protein Function: | eIF2-gamma: eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. |
| UniProt Protein Details: | Protein type:Translation initiation; Translation Chromosomal Location of Human Ortholog: Xp22.2-p22.1 Cellular Component: cytoplasm; cytosol; nucleus Molecular Function:protein binding; translation factor activity, nucleic acid binding; translation initiation factor activity Biological Process: formation of translation preinitiation complex; translational initiation; transmembrane transport |
| NCBI Summary: | The protein encoded by this gene is the largest subunit of a heterotrimeric GTP-binding protein involved in the recruitment of methionyl-tRNA(i) to the 40 S ribosomal subunit. [provided by RefSeq, Jan 2010] |
| UniProt Code: | P41091 |
| NCBI GenInfo Identifier: | 729816 |
| NCBI Gene ID: | 1968 |
| NCBI Accession: | P41091.3 |
| UniProt Secondary Accession: | P41091,Q53HK3, A0A024RBY4, A8K2Y2, B2R5N2, B5BTZ4, |
| UniProt Related Accession: | P41091 |
| Molecular Weight: | 51,109 Da |
| NCBI Full Name: | Eukaryotic translation initiation factor 2 subunit 3 |
| NCBI Synonym Full Names: | eukaryotic translation initiation factor 2 subunit gamma |
| NCBI Official Symbol: | EIF2S3 |
| NCBI Official Synonym Symbols: | EIF2; EIF2G; eIF-2gA; EIF2gamma |
| NCBI Protein Information: | eukaryotic translation initiation factor 2 subunit 3 |
| UniProt Protein Name: | Eukaryotic translation initiation factor 2 subunit 3 |
| UniProt Synonym Protein Names: | Eukaryotic translation initiation factor 2 subunit gamma X; eIF-2-gamma X; eIF-2gX |
| Protein Family: | Eukaryotic translation initiation factor |
| UniProt Gene Name: | EIF2S3 |
| UniProt Entry Name: | IF2G_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EIF2S3 antibody (CAB6581) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human liver using EIF2S3 antibody (CAB6581) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human stomach using EIF2S3 antibody (CAB6581) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat brain using EIF2S3 antibody (CAB6581) at dilution of 1:100 (40x lens). |
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