Metabolism Antibodies 2
Anti-EIF2S2 Antibody (CAB5894)
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- SKU:
- CAB5894
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-EIF2S2 Antibody |
| Antibody SKU: | CAB5894 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 134-333 of human EIF2S2 (NP_003899.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, SKOV3, HepG2, Mouse heart, Mouse spinal cord |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 134-333 of human EIF2S2 (NP_003899.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PDED EILE KDEA LEDE DNKK DDGI SFSN QTGP AWAG SERD YTYE ELLN RVFN IMRE KNPD MVAG EKRK FVMK PPQV VRVG TKKT SFVN FTDI CKLL HRQP KHLL AFLL AELG TSGS IDGN NQLV IKGR FQQK QIEN VLRR YIKE YVTC HTCR SPDT ILQK DTRL YFLQ CETC HSRC SVAS IKTG FQAV TGKR AQLR AKAN |
| Gene ID: | 8894 |
| Uniprot: | P20042 |
| Cellular Location: | |
| Calculated MW: | 38kDa |
| Observed MW: | 47kDa |
| Synonyms: | EIF2S2, EIF2, EIF2B, EIF2beta, PPP1R67, eIF-2-beta |
| Background: | Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | eIF2-beta: a translational regulatory protein that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. |
| UniProt Protein Details: | Protein type:Translation; Translation initiation Chromosomal Location of Human Ortholog: 20q11.2 Cellular Component: cytoplasm; nucleus; cytosol; eukaryotic translation initiation factor 2 complex Molecular Function:protein binding; RNA binding; translation factor activity, nucleic acid binding; metal ion binding; translation initiation factor activity Biological Process: cellular protein metabolic process; translation; in utero embryonic development; male gonad development; translational initiation; gene expression |
| NCBI Summary: | Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P20042 |
| NCBI GenInfo Identifier: | 12644154 |
| NCBI Gene ID: | 8894 |
| NCBI Accession: | P20042.2 |
| UniProt Secondary Accession: | P20042,Q9BVU0, Q9UJE4, |
| UniProt Related Accession: | P20042 |
| Molecular Weight: | 333 |
| NCBI Full Name: | Eukaryotic translation initiation factor 2 subunit 2 |
| NCBI Synonym Full Names: | eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa |
| NCBI Official Symbol: | EIF2S2 |
| NCBI Official Synonym Symbols: | EIF2; EIF2B; PPP1R67; EIF2beta; eIF-2-beta |
| NCBI Protein Information: | eukaryotic translation initiation factor 2 subunit 2; protein phosphatase 1, regulatory subunit 67; eukaryotic translation initiation factor 2 subunit beta |
| UniProt Protein Name: | Eukaryotic translation initiation factor 2 subunit 2 |
| UniProt Synonym Protein Names: | Eukaryotic translation initiation factor 2 subunit beta; eIF-2-beta |
| Protein Family: | Eukaryotic translation initiation factor |
| UniProt Gene Name: | EIF2S2 |
| UniProt Entry Name: | IF2B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EIF2S2 antibody (CAB5894) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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