Metabolism Antibodies 1

Anti-EIF2B3 Antibody (CAB10262)

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SKU:
CAB10262
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-EIF2B3 Antibody
Antibody SKU:CAB10262
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human EIF2B3 (NP_065098.1).
Application:WB IHC
Recommended Dilution:WB 1:1000 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, K-562, HT-29, Mouse heart, Mouse lung, Mouse brain, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human EIF2B3 (NP_065098.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEFQ AVVM AVGG GSRM TDLT SSIP KPLL PVGN KPLI WYPL NLLE RVGF EEVI VVTT RDVQ KALC AEFK MKMK PDIV CIPD DADM GTAD SLRY IYPK LKTD VLVL SCDL ITDV ALHE VVDL FRAY DASL AMLM RKGQ DSIE PVPG QKGK KKAV EQRD FIGV DSTG KRLL FMAN EADL DEEL VIKG SILQ KHPR IRFH TGLV DAHL YCLK KYIV DFLM ENGS ITSI RSEL IPYL VRKQ FSSA SSQQ GQEE KEED LKKK ELKS LDIY SFIK EA
Gene ID:8891
Uniprot:Q9NR50
Cellular Location:
Calculated MW:44kDa/46kDa/50kDa
Observed MW:50kDa
Synonyms:EIF2B3, EIF-2B, EIF2Bgamma
Background:The protein encoded by this gene is one of the subunits of initiation factor eIF2B, which catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. It has also been found to function as a cofactor of hepatitis C virus internal ribosome entry site-mediated translation. Mutations in this gene have been associated with leukodystrophy with vanishing white matter. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:eIF2B-gamma: Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. Defects in EIF2B3 are a cause of leukodystrophy with vanishing white matter (VWM). VWM is a leukodystrophy that occurs mainly in children. Neurological signs include progressive cerebellar ataxia, spasticity, inconstant optic atrophy and relatively preserved mental abilities. The disease is chronic-progressive with, in most individuals, additional episodes of rapid deterioration following febrile infections or minor head trauma. While childhood onset is the most common form of the disorder, some severe forms are apparent at birth. A severe, early-onset form seen among the Cree and Chippewayan populations of Quebec and Manitoba is called Cree leukoencephalopathy. Milder forms may not become evident until adolescence or adulthood. Some females with milder forms of the disease who survive to adolescence exhibit ovarian dysfunction. This variant of the disorder is called ovarioleukodystrophy. Belongs to the eIF-2B gamma/epsilon subunits family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Translation; Translation initiation

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: cytoplasm; cytosol; eukaryotic translation initiation factor 2B complex

Molecular Function:guanyl-nucleotide exchange factor activity; protein binding; translation factor activity, nucleic acid binding; translation initiation factor activity

Biological Process: cellular response to stimulus; negative regulation of translation initiation in response to stress; oligodendrocyte development; response to glucose stimulus; response to heat; response to peptide hormone stimulus; translational initiation

Disease: Leukoencephalopathy With Vanishing White Matter

NCBI Summary:The protein encoded by this gene is one of the subunits of initiation factor eIF2B, which catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. It has also been found to function as a cofactor of hepatitis C virus internal ribosome entry site-mediated translation. Mutations in this gene have been associated with leukodystrophy with vanishing white matter. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
UniProt Code:Q9NR50
NCBI GenInfo Identifier:18203317
NCBI Gene ID:8891
NCBI Accession:Q9NR50.1
UniProt Secondary Accession:Q9NR50,Q5QP89, Q5QP90, Q8NDB5, Q8WV57, Q9H850, B2RBH8 D3DPZ2,
UniProt Related Accession:Q9NR50
Molecular Weight:50kDa
NCBI Full Name:Translation initiation factor eIF-2B subunit gamma
NCBI Synonym Full Names:eukaryotic translation initiation factor 2B subunit gamma
NCBI Official Symbol:EIF2B3  
NCBI Official Synonym Symbols:EIF-2B; EIF2Bgamma  
NCBI Protein Information:translation initiation factor eIF-2B subunit gamma
UniProt Protein Name:Translation initiation factor eIF-2B subunit gamma
UniProt Synonym Protein Names:eIF-2B GDP-GTP exchange factor subunit gamma
Protein Family:Translation initiation factor
UniProt Gene Name:EIF2B3  
Anti-EIF2B3 Antibody (CAB10262)Western blot analysis of extracts of various cell lines, using EIF2B3 antibody (CAB10262) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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