Name: Anti-EIF2B1 Antibody (CAB7892)
Brand: Metabolism Antibodies 2
SKU: CAB7892
Availability: OutOfStock

Antibody Name:	Anti-EIF2B1 Antibody
Antibody SKU:	CAB7892
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-305 of human EIF2B1 (NP_001405.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	SH-SY5Y, MCF7, SKOV3, NCI-H460, Mouse liver, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-305 of human EIF2B1 (NP_001405.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDDK ELIE YFKS QMKE DPDM ASAV AAIR TLLE FLKR DKGE TIQG LRAN LTSA IETL CGVD SSVA VSSG GELF LRFI SLAS LEYS DYSK CKKI MIER GELF LRRI SLSR NKIA DLCH TFIK DGAT ILTH AYSR VVLR VLEA AVAA KKRF SVYV TESQ PDLS GKKM AKAL CHLN VPVT VVLD AAVG YIME KADL VIVG AEGV VENG GIIN KIGT NQMA VCAK AQNK PFYV VAES FKFV RLFP LNQQ DVPD KFKY KADT LKVA QTGQ DLKE EHPW VDYT APSL ITLL FTDL GVLT PSAV SDEL IKLY L
Gene ID:	1967
Uniprot:	Q14232
Cellular Location:
Calculated MW:	24kDa/33kDa
Observed MW:	34kDa

Synonyms:	EIF2B1, EIF2B, EIF2BA
Background:	This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter.

UniProt Protein Function:	eIF2B-alpha: Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. Defects in EIF2B1 are a cause of leukodystrophy with vanishing white matter (VWM). VWM is a leukodystrophy that occurs mainly in children. Neurological signs include progressive cerebellar ataxia, spasticity, inconstant optic atrophy and relatively preserved mental abilities. The disease is chronic-progressive with, in most individuals, additional episodes of rapid deterioration following febrile infections or minor head trauma. While childhood onset is the most common form of the disorder, some severe forms are apparent at birth. A severe, early-onset form seen among the Cree and Chippewayan populations of Quebec and Manitoba is called Cree leukoencephalopathy. Milder forms may not become evident until adolescence or adulthood. Some females with milder forms of the disease who survive to adolescence exhibit ovarian dysfunction. This variant of the disorder is called ovarioleukodystrophy. Belongs to the eIF-2B alpha/beta/delta subunits family.
UniProt Protein Details:	Protein type:Translation; Translation initiation Chromosomal Location of Human Ortholog: 12q24.31 Cellular Component: cytoplasm; cytosol; eukaryotic translation initiation factor 2B complex; membrane; plasma membrane Molecular Function:guanyl-nucleotide exchange factor activity; protein binding; S-methyl-5-thioribose-1-phosphate isomerase activity; translation initiation factor activity Biological Process: cellular response to stimulus; methionine salvage; oligodendrocyte development; regulation of translational initiation; response to glucose stimulus; response to heat; response to peptide hormone stimulus; translational initiation Disease: Leukoencephalopathy With Vanishing White Matter
NCBI Summary:	This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. [provided by RefSeq, Oct 2009]
UniProt Code:	Q14232
NCBI GenInfo Identifier:	2494303
NCBI Gene ID:	1967
NCBI Accession:	Q14232.1
UniProt Secondary Accession:	Q14232,Q3SXP4, A6NLY9, B4DGX0,
UniProt Related Accession:	Q14232
Molecular Weight:	24,618 Da
NCBI Full Name:	Translation initiation factor eIF-2B subunit alpha
NCBI Synonym Full Names:	eukaryotic translation initiation factor 2B subunit alpha
NCBI Official Symbol:	EIF2B1
NCBI Official Synonym Symbols:	EIF2B; EIF2BA
NCBI Protein Information:	translation initiation factor eIF-2B subunit alpha
UniProt Protein Name:	Translation initiation factor eIF-2B subunit alpha
UniProt Synonym Protein Names:	eIF-2B GDP-GTP exchange factor subunit alpha
Protein Family:	Translation initiation factor
UniProt Gene Name:	EIF2B1
UniProt Entry Name:	EI2BA_HUMAN

	Western blot analysis of extracts of various cell lines, using EIF2B1 antibody (CAB7892) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using EIF2B1 antibody (CAB7892) at dilution of 1:100. Blue: DAPI for nuclear staining.