Metabolism Antibodies 1

Anti-eIF2Alpha Antibody (CAB0764)

(No reviews yet) Write a Review
SKU:
CAB0764
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-eIF2Alpha Antibody
Antibody SKU:CAB0764
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human eIF2Alpha (NP_004085.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:1000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:LO2, Jurkat, MCF7, SW480, SKOV3, Mouse kidney, Mouse spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human eIF2Alpha (NP_004085.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPGL SCRF YQHK FPEV EDVV MVNV RSIA EMGA YVSL LEYN NIEG MILL SELS RRRI RSIN KLIR IGRN ECVV VIRV DKEK GYID LSKR RVSP EEAI KCED KFTK SKTV YSIL RHVA EVLE YTKD EQLE SLFQ RTAW VFDD KYKR PGYG AYDA FKHA VSDP SILD SLDL NEDE REVL INNI NRRL TPQA VKIR ADIE VACY GYEG IDAV KEAL RAGL NCST ENMP IKIN LIAP PRYV MTTT TLER TEGL SVLS QAMA VIKE
Gene ID:1965
Uniprot:P05198
Cellular Location:Cytoplasmic granule
Calculated MW:36kDa
Observed MW:36kDa
Synonyms:EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A, EIF2S1
Background:The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
UniProt Protein Function:eIF2-alpha: a translation initiation factor that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40s ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. Phosphorylated by at least 4 kinases: PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. Upregulated in some thyroid cancers and bronchiolo-alveolar adenocarcinomas; aberrant phosphorylation correlates with Alzheimer disease and Epstein-Barr virus infections.
UniProt Protein Details:

Protein type:RNA-binding; Translation initiation; Translation

Chromosomal Location of Human Ortholog: 14q23.3

Cellular Component: eukaryotic translation initiation factor 2B complex; polysome; membrane; stress granule; nucleus; cytosol; eukaryotic translation initiation factor 2 complex

Molecular Function:protein binding; translation initiation factor activity; ribosome binding

Biological Process: regulation of translation initiation in response to stress; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; translation; unfolded protein response; protein amino acid autophosphorylation; translational initiation; gene expression

NCBI Summary:The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
UniProt Code:P05198
NCBI GenInfo Identifier:124200
NCBI Gene ID:1965
NCBI Accession:P05198.3
UniProt Related Accession:P05198
Molecular Weight:315
NCBI Full Name:Eukaryotic translation initiation factor 2 subunit 1
NCBI Synonym Full Names:eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
NCBI Official Symbol:EIF2S1  
NCBI Official Synonym Symbols:EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha  
NCBI Protein Information:eukaryotic translation initiation factor 2 subunit 1; eIF-2-alpha; eukaryotic translation initiation factor 2 subunit alpha
UniProt Protein Name:Eukaryotic translation initiation factor 2 subunit 1
UniProt Synonym Protein Names:Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha
Protein Family:Eukaryotic translation initiation factor
UniProt Gene Name:EIF2S1  
UniProt Entry Name:IF2A_HUMAN
Anti-eIF2Alpha Antibody (CAB0764)Western blot analysis of extracts of various cell lines, using eIF2a antibody (CAB0764) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-eIF2Alpha Antibody (CAB0764)Immunohistochemistry of paraffin-embedded rat heart using eIF2a antibody (CAB0764) at dilution of 1:100 (40x lens).
Anti-eIF2Alpha Antibody (CAB0764)Immunohistochemistry of paraffin-embedded human liver cancer using eIF2a antibody (CAB0764) at dilution of 1:100 (40x lens).
Anti-eIF2Alpha Antibody (CAB0764)Immunohistochemistry of paraffin-embedded mouse heart using eIF2a antibody (CAB0764) at dilution of 1:100 (40x lens).
Anti-eIF2Alpha Antibody (CAB0764)Immunofluorescence analysis of PC-12 cells using eIF2a antibody (CAB0764) at dilution of 1:100. Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products