Antibody Name:	Anti-Eif2ak2 Antibody [KO Validated]
Antibody SKU:	CAB5578
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human Eif2ak2 (NP_002750.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Jurkat

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human Eif2ak2 (NP_002750.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAGD LSAG FFME ELNT YRQK QGVV LKYQ ELPN SGPP HDRR FTFQ VIID GREF PEGE GRSK KEAK NAAA KLAV EILN KEKK AVSP LLLT TTNS SEGL SMGN YIGL INRI AQKK RLTV NYEQ CASG VHGP EGFH YKCK MGQK EYSI GTGS TKQE AKQL AAKL AYLQ ILSE ETSV KSDY LSSG SFAT TCES QSNS LVTS TLAS ESSS EGDF SADT SEIN SNSD SLNS SSLL MNGL RNNQ RKAK RSLA PRFD LPDM KETK YTVD KRFG MDFK EI
Gene ID:	5610
Uniprot:	P19525
Cellular Location:	Cytoplasm, Nucleus, perinuclear region
Calculated MW:	57kDa/62kDa
Observed MW:	74kDa

Synonyms:

EIF2AK1, PKR, PPP1R83, PRKR, Eif2ak2, EIF2AK2

Background:

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene.

UniProt Protein Function:	PKR: a protein kinase of the PEK family. Upon binding double-stranded RNA, it becomes autophosphorylated and activated. Phosphorylates and inhibits the alpha subunit of eIF2 alpha, which leads to an inhibition of the initiation of protein synthesis. Controls the activation of several transcription factors such as NF-kappaB, p53 and Stats. Mediates apoptosis induced by many different stimuli, such as LPS, TNF-alpha, viral infection and serum starvation.
UniProt Protein Details:	Protein type:Translation; Kinase, protein; EC 2.7.10.2; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Other group; PEK family Chromosomal Location of Human Ortholog: 2p22-p21 Cellular Component: membrane; perinuclear region of cytoplasm; cytoplasm; ribosome; nucleus; cytosol Molecular Function:protein serine/threonine kinase activity; protein binding; double-stranded RNA binding; non-membrane spanning protein tyrosine kinase activity; protein phosphatase type 2A regulator activity; ATP binding; eukaryotic translation initiation factor 2alpha kinase activity; protein kinase activity Biological Process: positive regulation of cytokine production; peptidyl-tyrosine phosphorylation; translation; activation of MAPKK activity; transcription, DNA-dependent; unfolded protein response; response to virus; protein amino acid autophosphorylation; viral infectious cycle; protein amino acid phosphorylation; positive regulation of stress-activated MAPK cascade; positive regulation of chemokine production; evasion by virus of host immune response; activation of NF-kappaB transcription factor; negative regulation of cell proliferation; modification by virus of host cellular process; negative regulation of viral genome replication; virus-host interaction; negative regulation of translation; innate immune response; negative regulation of osteoblast proliferation; defense response to virus; negative regulation of apoptosis
NCBI Summary:	The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
UniProt Code:	P19525
NCBI GenInfo Identifier:	125527
NCBI Gene ID:	5610
NCBI Accession:	P19525.2
UniProt Secondary Accession:	P19525,Q52M43, Q7Z6F6, Q9UIR4, A8K3P0, D6W584, E9PC80
UniProt Related Accession:	P19525
Molecular Weight:	62,094 Da
NCBI Full Name:	Interferon-induced, double-stranded RNA-activated protein kinase
NCBI Synonym Full Names:	eukaryotic translation initiation factor 2-alpha kinase 2
NCBI Official Symbol:	EIF2AK2
NCBI Official Synonym Symbols:	PKR; PRKR; EIF2AK1; PPP1R83
NCBI Protein Information:	interferon-induced, double-stranded RNA-activated protein kinase; p68 kinase; eIF-2A protein kinase 2; P1/eIF-2A protein kinase; tyrosine-protein kinase EIF2AK2; interferon-inducible elF2alpha kinase; double stranded RNA activated protein kinase; protein phosphatase 1, regulatory subunit 83; protein kinase, interferon-inducible double stranded RNA dependent
UniProt Protein Name:	Interferon-induced, double-stranded RNA-activated protein kinase
UniProt Synonym Protein Names:	Eukaryotic translation initiation factor 2-alpha kinase 2; eIF-2A protein kinase 2; Interferon-inducible RNA-dependent protein kinase; P1/eIF-2A protein kinase; Protein kinase RNA-activated; PKR; Tyrosine-protein kinase EIF2AK2 (EC:2.7.10.2); p68 kinase
UniProt Gene Name:	EIF2AK2
UniProt Entry Name:	E2AK2_HUMAN

	Western blot analysis of extracts of various cell lines, using Eif2ak2 antibody (CAB5578) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human liver using Eif2ak2 antibody (CAB5578) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using EIF2AK2 antibody (CAB5578) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-Phospho-EIF2AK2-T451 Antibody (CABP0679)	Human Interferon-induced, double-stranded RNA-activated protein kinase (EIF2AK2) ELISA Kit
Anti-PKR Antibody (CAB19545)	PKR (Phospho-Thr446) Colorimetric Cell-Based ELISA Kit
Anti-Phospho-EIF2AK2-T446 Antibody (CABP1134)	PKR (Phospho-Thr258) Colorimetric Cell-Based ELISA Kit
EIF2AK2 Antibody (PACO03025)	PKR Colorimetric Cell-Based ELISA Kit
Phospho-EIF2AK2 (T446) Antibody (PACO04769)