Name: Anti-EIF2AK1 Antibody (CAB3415)
Brand: Cell Biology Antibodies 8
SKU: CAB3415
Availability: OutOfStock

Antibody Name:	Anti-EIF2AK1 Antibody
Antibody SKU:	CAB3415
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 371-630 of human EIF2AK1 (NP_055228.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	SH-SY5Y, HeLa, HL-60, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 371-630 of human EIF2AK1 (NP_055228.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QTEA QYHL MLHI QMQL CELS LWDW IVER NKRG REYV DESA CPYV MANV ATKI FQEL VEGV FYIH NMGI VHRD LKPR NIFL HGPD QQVK IGDF GLAC TDIL QKNT DWTN RNGK RTPT HTSR VGTC LYAS PEQL EGSE YDAK SDMY SLGV VLLE LFQP FGTE MERA EVLT GLRT GQLP ESLR KRCP VQAK YIQH LTRR NSSQ RPSA IQLL QSEL FQNS GNVN LTLQ MKII EQEK EIAE LKKQ LNLL SQDK GVRD DGKD GGVG
Gene ID:	27102
Uniprot:	Q9BQI3
Cellular Location:	Cytoplasm
Calculated MW:	71kDa
Observed MW:	71kDa

Synonyms:	EIF2AK1, HCR, HRI
Background:	The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	HRI: a protein kinase of the PEK family. Phosphorylates the alpha subunit of eukaryotic initiation factor 2 in response to various stress conditions, inhibiting protein synthesis at the level of initiation. Its kinase activity is induced by low levels of heme and inhibited by the presence of heme. Binding of nitric oxide (NO) to the heme iron in the N-terminal heme-binding domain activates the kinase activity, while binding of carbon monoxide (CO) suppresses kinase activity. Requires association with a multiprotein complex containing Hsp90, CDC37 and PPP5C for maturation and activation by autophosporylation. Two alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:Translation; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; EC 2.7.11.1; Kinase, protein; Other group; PEK family Chromosomal Location of Human Ortholog: 7p22 Cellular Component: cytoplasm Molecular Function:protein homodimerization activity; heme binding; ATP binding; eukaryotic translation initiation factor 2alpha kinase activity Biological Process: response to external stimulus; negative regulation of cell proliferation; negative regulation of translational initiation by iron; protoporphyrinogen IX metabolic process; protein amino acid autophosphorylation; response to stress; negative regulation of hemoglobin biosynthetic process
NCBI Summary:	The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
UniProt Code:	Q9BQI3
NCBI GenInfo Identifier:	32172458
NCBI Gene ID:	27102
NCBI Accession:	Q9BQI3.2
UniProt Secondary Accession:	Q9BQI3,Q549K6, Q8NBW3, Q9HC02, Q9NYE0, Q9P0V6, Q9P1J5 Q9P2H8, Q9UHG4, A8K2R2,
UniProt Related Accession:	Q9BQI3
Molecular Weight:
NCBI Full Name:	Eukaryotic translation initiation factor 2-alpha kinase 1
NCBI Synonym Full Names:	eukaryotic translation initiation factor 2-alpha kinase 1
NCBI Official Symbol:	EIF2AK1
NCBI Official Synonym Symbols:	HCR; HRI
NCBI Protein Information:	eukaryotic translation initiation factor 2-alpha kinase 1; heme-regulated inhibitor; heme-regulated repressor; heme-controlled repressor; heme sensitive initiation factor 2a kinase; heme regulated initiation factor 2 alpha kinase; heme-regulated initiation factor 2-alpha kinase; hemin-sensitive initiation factor 2-alpha kinase; heme-regulated eukaryotic initiation factor eIF-2-alpha kinase
UniProt Protein Name:	Eukaryotic translation initiation factor 2-alpha kinase 1
UniProt Synonym Protein Names:	Heme-controlled repressor; HCR; Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; Heme-regulated inhibitor; Hemin-sensitive initiation factor 2-alpha kinase
Protein Family:	Eukaryotic translation initiation factor 2-alpha kinase
UniProt Gene Name:	EIF2AK1
UniProt Entry Name:	E2AK1_HUMAN

Western blot analysis of extracts of various cell lines, using EIF2AK1 antibody (CAB3415) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Antibodies

Anti-EIF2AK1 Antibody (CAB14119)

EIF2AK1 Antibody (PACO00937)

EIF2AK1 Antibody (PACO09031)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)