Metabolism Antibodies 3
Anti-EIF2A Antibody (CAB16205)
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- SKU:
- CAB16205
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-EIF2A Antibody |
| Antibody SKU: | CAB16205 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide of human EIF2A |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | U-87MG |
| Immunogen: | A synthetic peptide of human EIF2A |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 83939 |
| Uniprot: | Q9BY44 |
| Cellular Location: | |
| Calculated MW: | 41kDa/57kDa/62kDa/64kDa |
| Observed MW: | 65kDa |
| Synonyms: | EIF2A, CDA02, EIF-2A, MST089, MSTP004, MSTP089 |
| Background: | This gene encodes a eukaryotic translation initiation factor that catalyzes the formation of puromycin-sensitive 80 S preinitiation complexes and the poly(U)-directed synthesis of polyphenylalanine at low concentrations of Mg2+. This gene should not be confused with eIF2-alpha (EIF2S1, Gene ID: 1965), the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40 S ribosomal subunit, the encoded protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner. |
| NCBI Summary: | This gene encodes a eukaryotic translation initiation factor that catalyzes the formation of puromycin-sensitive 80 S preinitiation complexes and the poly(U)-directed synthesis of polyphenylalanine at low concentrations of Mg2+. This gene should not be confused with eIF2-alpha (EIF2S1, Gene ID: 1965), the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40 S ribosomal subunit, the encoded protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016] |
| UniProt Code: | Q9BY44 |
| NCBI GenInfo Identifier: | 209572747 |
| NCBI Gene ID: | 83939 |
| NCBI Accession: | Q9BY44.3 |
| UniProt Secondary Accession: | Q9BY44,Q5QTR2, Q7Z4E9, Q8NFM1, Q96EW9, Q96K81, A8MPS6 B4DF96, B4DQ14, D3DNI9, |
| UniProt Related Accession: | Q9BY44 |
| Molecular Weight: | 62 kDa |
| NCBI Full Name: | Eukaryotic translation initiation factor 2A |
| NCBI Synonym Full Names: | eukaryotic translation initiation factor 2A |
| NCBI Official Symbol: | EIF2A |
| NCBI Official Synonym Symbols: | CDA02; EIF-2A; MST089; MSTP004; MSTP089 |
| NCBI Protein Information: | eukaryotic translation initiation factor 2A |
| UniProt Protein Name: | Eukaryotic translation initiation factor 2A |
| UniProt Synonym Protein Names: | 65 kDa eukaryotic translation initiation factor 2A |
| Protein Family: | Eukaryotic translation initiation factor |
| UniProt Gene Name: | EIF2A |
 | Western blot analysis of extracts of U-87MG cells, using EIF2A antibody (CAB16205) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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