Metabolism Antibodies 2
Anti-EIF1AX Antibody (CAB5917)
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- SKU:
- CAB5917
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-EIF1AX Antibody |
| Antibody SKU: | CAB5917 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 10-144 of human EIF1AX (NP_001403.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SKOV3, HT-29, MCF7, NCI-H460, Mouse brain, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 10-144 of human EIF1AX (NP_001403.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KNRR RGKN ENES EKRE LVFK EDGQ EYAQ VIKM LGNG RLEA MCFD GVKR LCHI RGKL RKKV WINT SDII LVGL RDYQ DNKA DVIL KYNA DEAR SLKA YGEL PEHA KINE TDTF GPGD DDEI QFDD IGDD DEDI DDI |
| Gene ID: | 1964 |
| Uniprot: | P47813 |
| Cellular Location: | |
| Calculated MW: | 16kDa |
| Observed MW: | 16kDa |
| Synonyms: | EIF1AX, EIF1A, EIF1AP1, EIF4C, eIF-1A, eIF-4C, X-linked |
| Background: | This gene encodes an essential eukaryotic translation initiation factor. The protein is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA. |
| UniProt Protein Function: | EIF1AX: Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits. Belongs to the eIF-1A family |
| UniProt Protein Details: | Protein type:Translation initiation; RNA-binding; Translation Chromosomal Location of Human Ortholog: Xp22.12 Cellular Component: cytosol Molecular Function:protein binding; translation factor activity, nucleic acid binding; translation initiation factor activity Biological Process: cellular protein metabolic process; translation; translational initiation; gene expression |
| NCBI Summary: | This gene encodes an essential eukaryotic translation initiation factor. The protein is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P47813 |
| NCBI GenInfo Identifier: | 1352425 |
| NCBI Gene ID: | 1964 |
| NCBI Accession: | P47813.2 |
| UniProt Secondary Accession: | P47813,Q0VGC2, Q5JPS5, Q5JPS6, B2R5U5, |
| UniProt Related Accession: | P47813 |
| Molecular Weight: | Calculated: 16kDaObserved: 16kDa |
| NCBI Full Name: | Eukaryotic translation initiation factor 1A, X-chromosomal |
| NCBI Synonym Full Names: | eukaryotic translation initiation factor 1A, X-linked |
| NCBI Official Symbol: | EIF1AX |
| NCBI Official Synonym Symbols: | EIF1A; EIF4C; eIF-1A; eIF-4C; EIF1AP1 |
| NCBI Protein Information: | eukaryotic translation initiation factor 1A, X-chromosomal; eIF-1A X isoform; eukaryotic translation initiation factor 4C; Putative eukaryotic translation initiation factor 1A; eukaryotic translation initiation factor 1A, X chromosome |
| UniProt Protein Name: | Eukaryotic translation initiation factor 1A, X-chromosomal |
| UniProt Synonym Protein Names: | Eukaryotic translation initiation factor 4C; eIF-4C |
| Protein Family: | Eukaryotic translation initiation factor |
| UniProt Gene Name: | EIF1AX |
| UniProt Entry Name: | IF1AX_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EIF1AX antibody (CAB5917) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human stomach using EIF1AX antibody (CAB5917) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using EIF1AX antibody (CAB5917) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using EIF1AX Polyclonal Antibody (CAB5917) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using EIF1AX Polyclonal Antibody (CAB5917) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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