Cell Biology Antibodies 12

Anti-EHMT1 Antibody (CAB8513)

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SKU:
CAB8513
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-EHMT1 Antibody
Antibody SKU:CAB8513
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human EHMT1 (NP_079033.4).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Raji, 293T, HepG2, BT-474, Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human EHMT1 (NP_079033.4).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAAA DAEA VPAR GEPQ QDCC VKTE LLGE ETPM AADE GSAE KQAG EAHM AADG ETNG SCEN SDAS SHAN AAKH TQDS ARVN PQDG TNTL TRIA ENGV SERD SEAA KQNH VTAD DFVQ TSVI GSNG YILN KPAL QAQP LRTT STLA SSLP GHAA KTLP GGAG KGRT PSAF PQTP AAPP ATLG EGSA DTED RKLP APGA DVKV HRAR KTMP KSVV GLHA ASKD PREV REAR DHKE PKEE INKN ISDF GRQQ LLPP FPSL HQSL
Gene ID:79813
Uniprot:Q9H9B1
Cellular Location:Chromosome, Nucleus
Calculated MW:7kDa/86kDa/128kDa/141kDa
Observed MW:141kDa
Synonyms:EHMT1, EUHMTASE1, Eu-HMTase1, FP13812, GLP, GLP1, KMT1D, bA188C12.1
Background:The protein encoded by this gene is a histone methyltransferase that is part of the E2F6 complex, which represses transcription. The encoded protein methylates the Lys-9 position of histone H3, which tags it for transcriptional repression. This protein may be involved in the silencing of MYC- and E2F-responsive genes and therefore could play a role in the G0/G1 cell cycle transition. Defects in this gene are a cause of chromosome 9q subtelomeric deletion syndrome (9q-syndrome, also known as Kleefstra syndrome). Alternative splicing results in multiple transcript variants.
UniProt Protein Function:EHMT1: Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non- histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Defects in EHMT1 are the cause of chromosome 9q subtelomeric deletion syndrome (9q- syndrome). Common features seen in these patients are severe mental retardation, hypotonia, brachy(micro)cephaly, epileptic seizures, flat face with hypertelorism, synophrys, anteverted nares, cupid bow or tented upper lip, everted lower lip, prognathism, macroglossia, conotruncal heart defects, and behavioral problems. Belongs to the histone-lysine methyltransferase family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Methyltransferase; Methyltransferase, protein lysine; Amino Acid Metabolism - lysine degradation; EC 2.1.1.43

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: nucleoplasm; nucleus

Molecular Function:histone lysine N-methyltransferase activity (H3-K27 specific); histone lysine N-methyltransferase activity (H3-K9 specific); histone-lysine N-methyltransferase activity; methyltransferase activity; p53 binding; protein binding; protein-lysine N-methyltransferase activity

Biological Process: chromatin modification; DNA methylation; embryonic development; histone methylation; negative regulation of transcription, DNA-dependent; peptidyl-lysine di-methylation; peptidyl-lysine mono-methylation

Disease: Kleefstra Syndrome

NCBI Summary:The protein encoded by this gene is a histone methyltransferase that is part of the E2F6 complex, which represses transcription. The encoded protein methylates the Lys-9 position of histone H3, which tags it for transcriptional repression. This protein may be involved in the silencing of MYC- and E2F-responsive genes and therefore could play a role in the G0/G1 cell cycle transition. Defects in this gene are a cause of chromosome 9q subtelomeric deletion syndrome (9q-syndrome, also known as Kleefstra syndrome). Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
UniProt Code:Q9H9B1
NCBI GenInfo Identifier:325511404
NCBI Gene ID:79813
NCBI Accession:Q9H9B1.4
UniProt Secondary Accession:Q9H9B1,Q86X08, Q8TCN7, Q96F53, Q96JF1, Q96KH4, B1AQ58 B1AQ59,
UniProt Related Accession:Q9H9B1
Molecular Weight:86,704 Da
NCBI Full Name:Histone-lysine N-methyltransferase EHMT1
NCBI Synonym Full Names:euchromatic histone lysine methyltransferase 1
NCBI Official Symbol:EHMT1  
NCBI Official Synonym Symbols:GLP; GLP1; KMT1D; FP13812; EUHMTASE1; Eu-HMTase1; bA188C12.1  
NCBI Protein Information:histone-lysine N-methyltransferase EHMT1
UniProt Protein Name:Histone-lysine N-methyltransferase EHMT1
UniProt Synonym Protein Names:Euchromatic histone-lysine N-methyltransferase 1; Eu-HMTase1; G9a-like protein 1; GLP; GLP1; Histone H3-K9 methyltransferase 5; H3-K9-HMTase 5; Lysine N-methyltransferase 1D
Protein Family:Histone-lysine N-methyltransferase
UniProt Gene Name:EHMT1  
UniProt Entry Name:EHMT1_HUMAN
Anti-EHMT1 Polyclonal Antibody (CAB8513)Western blot analysis of extracts of various cell lines, using EHMT1 antibody (CAB8513) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
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