Cell Biology Antibodies 4
Anti-EHD4 Antibody (CAB13766)
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- SKU:
- CAB13766
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-EHD4 Antibody |
| Antibody SKU: | CAB13766 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 362-541 of human EHD4 (NP_644670.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa, 293T, Mouse brain, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 362-541 of human EHD4 (NP_644670.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MQEQ LENY DFTK FHSL KPKL IEAV DNML SNKI SPLM NLIS QEET STPT QLVQ GGAF DGTT EGPF NQGY GEGA KEGA DEEE WVVA KDKP VYDE LFYT LSPI NGKI SGVN AKKE MVTS KLPN SVLG KIWK LADC DCDG MLDE EEFA LAKH LIKI KLDG YELP SSLP PHLV PPSH RKSL PKAD |
| Gene ID: | 30844 |
| Uniprot: | Q9H223 |
| Cellular Location: | Cell membrane, Cytoplasmic side, Early endosome membrane, Peripheral membrane protein, Recycling endosome membrane |
| Calculated MW: | 61kDa |
| Observed MW: | 72kDa |
| Synonyms: | EHD4, PAST4 |
| Background: |
| UniProt Protein Function: | EHD4: an extracellular matrix protein that associates with type VI collagen. Contains 1 EF-hand calcium-binding domain and an EH domain. Highly expressed in heart and pancreas. |
| UniProt Protein Details: | Protein type:Extracellular matrix Chromosomal Location of Human Ortholog: 15q11.1 Cellular Component: early endosome membrane; endoplasmic reticulum; membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; recycling endosome membrane Molecular Function:ATP binding; calcium ion binding; GTP binding; nucleic acid binding; protein binding Biological Process: endocytic recycling; pinocytosis; positive regulation of peptidyl-tyrosine phosphorylation; protein homooligomerization; regulation of endocytosis |
| UniProt Code: | Q9H223 |
| NCBI GenInfo Identifier: | 18202935 |
| NCBI Gene ID: | 30844 |
| NCBI Accession: | Q9H223.1 |
| UniProt Secondary Accession: | Q9H223,Q9HAR1, Q9NZN2, |
| UniProt Related Accession: | Q9H223 |
| Molecular Weight: | 61,175 Da |
| NCBI Full Name: | EH domain-containing protein 4 |
| NCBI Synonym Full Names: | EH domain containing 4 |
| NCBI Official Symbol: | EHD4 |
| NCBI Official Synonym Symbols: | PAST4 |
| NCBI Protein Information: | EH domain-containing protein 4 |
| UniProt Protein Name: | EH domain-containing protein 4 |
| UniProt Synonym Protein Names: | Hepatocellular carcinoma-associated protein 10/11 |
| Protein Family: | EH domain-containing protein |
| UniProt Gene Name: | EHD4 |
| UniProt Entry Name: | EHD4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EHD4 antibody (CAB13766) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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