Name: Anti-EHD2 Antibody (CAB12945)
Brand: Cell Biology Antibodies 3
SKU: CAB12945
Availability: OutOfStock

Antibody Name:	Anti-EHD2 Antibody
Antibody SKU:	CAB12945
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 244-543 of human EHD2 (NP_055416.2).

Application:	WB
Recommended Dilution:	WB 1:1000 - 1:3000
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, U-87MG, HeLa, Mouse lung, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 244-543 of human EHD2 (NP_055416.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VGTP EVLR VYIG SFWS QPLL VPDN RRLF ELEE QDLF RDIQ GLPR HAAL RKLN DLVK RARL VRVH AYII SYLK KEMP SVFG KENK KKQL ILKL PVIF AKIQ LEHH ISPG DFPD CQKM QELL MAHD FTKF HSLK PKLL EALD EMLT HDIA KLMP LLRQ EELE STEV GVQG GAFE GTHM GPFV ERGP DEAM EDGE EGSD DEAE WVVT KDKS KYDE IFYN LAPA DGKL SGSK AKTW MVGT KLPN SVLG RIWK LSDV DRDG MLDD EEFA LASH LIEA KLEG HGLP ANLP RRLV PPSK RRHK GSAE
Gene ID:	30846
Uniprot:	Q9NZN4
Cellular Location:	Cell membrane, Cytoplasm, Cytoplasmic side, Endosome membrane, Membrane, Peripheral membrane protein, caveola, cytosol
Calculated MW:	46kDa/61kDa
Observed MW:	61kDa

Synonyms:

EHD2, PAST2

Background:

This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway.

UniProt Protein Function:	EHD2: Plays a role in membrane reorganization in response to nucleotide hydrolysis. Binds to liposomes and deforms them into tubules. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for normal fusion of myoblasts to skeletal muscle myotubes. Binds ATP; does not bind GTP.
UniProt Protein Details:	Protein type:Vesicle Chromosomal Location of Human Ortholog: 19q13.3 Cellular Component: caveola; cytosol; endosome membrane; extrinsic to membrane; nucleus; plasma membrane; recycling endosome membrane Molecular Function:nucleic acid binding; protein binding Biological Process: blood coagulation; endocytic recycling
NCBI Summary:	This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9NZN4
NCBI GenInfo Identifier:	57015322
NCBI Gene ID:	30846
NCBI Accession:	Q9NZN4.2
UniProt Secondary Accession:	Q9NZN4,Q96CB6, B2RDH9, B4DNU6,
UniProt Related Accession:	Q9NZN4
Molecular Weight:	46,059 Da
NCBI Full Name:	EH domain-containing protein 2
NCBI Synonym Full Names:	EH domain containing 2
NCBI Official Symbol:	EHD2
NCBI Official Synonym Symbols:	PAST2
NCBI Protein Information:	EH domain-containing protein 2
UniProt Protein Name:	EH domain-containing protein 2
UniProt Synonym Protein Names:	PAST homolog 2Curated
Protein Family:	EH domain-containing protein
UniProt Gene Name:	EHD2
UniProt Entry Name:	EHD2_HUMAN

Western blot analysis of extracts of various cell lines, using EHD2 antibody (CAB12945) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Antibodies

EHD2 Antibody (PACO09021)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)