Epigenetics & Nuclear Signaling Antibodies 2
Anti-Egr4 Antibody (CAB16369)
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- SKU:
- CAB16369
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-Egr4 Antibody |
| Antibody SKU: | CAB16369 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-367 of mouse Egr4 (NP_065621.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse brain, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-367 of mouse Egr4 (NP_065621.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MLHL SDFS GSDA LLSK PTEG CAHA SPEL PRLP ARDA PSAA AYPG GDFL SWAL SSCG AGGD LTDS CFLE GPAP TPPS GLSY SGSF FIQA VPEH PHDP EALF NLMS GILG LAPF PGPE AAAS RSPL DVPF PAGP DALL PDLY SPDL SSAA FPEA FWEA APSA GAPS QCLF EPQL SPPD VKPG LRAP PASP ALDA AASA FKGP YAPW ELLS AGVP GNCG SQGS FQTT PEAR FSAV GTKV EDLL SISC PAEL PGPA ARLY QAGA YDTF SLAP GDLG EGTE GLPA LLTP PGGE GGSG GEGG EFLA APPA QLSP LGLR GAAT ADFS KPLV ADLP GGSG VAAP SSPA ASFP AAKA RRKG RRGG KCSA RCFC PRP |
| Gene ID: | 13656 |
| Uniprot: | Q05215 |
| Cellular Location: | Nucleus |
| Calculated MW: | 61kDa |
| Observed MW: | 58kDa |
| Synonyms: | EGR4, NGFI-C, NGFIC, PAT133, NGF1-C, pAT133, Egr4 |
| Background: |
| UniProt Protein Function: | EGR4: Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-GCGGGGGCG-3' (GSG). Activates the transcription of target genes whose products are required for mitogenesis and differentiation. Belongs to the EGR C2H2-type zinc-finger protein family. |
| UniProt Protein Details: | Protein type:C2H2-type zinc finger protein; DNA-binding Chromosomal Location of Human Ortholog: 2p13 Cellular Component: cytoplasm; nucleus Molecular Function:DNA binding; metal ion binding; transcription factor activity Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; negative regulation of apoptosis |
| UniProt Code: | Q05215 |
| NCBI GenInfo Identifier: | 485956294 |
| NCBI Gene ID: | 1961 |
| NCBI Accession: | Q05215.3 |
| UniProt Secondary Accession: | Q05215,Q2Z1P5, B2RAE3, G3V1T5, |
| UniProt Related Accession: | Q05215 |
| Molecular Weight: | 589 |
| NCBI Full Name: | Early growth response protein 4 |
| NCBI Synonym Full Names: | early growth response 4 |
| NCBI Official Symbol: | EGR4 |
| NCBI Official Synonym Symbols: | NGFIC; NGFI-C; PAT133 |
| NCBI Protein Information: | early growth response protein 4; AT133; EGR-4 |
| UniProt Protein Name: | Early growth response protein 4 |
| UniProt Synonym Protein Names: | AT133 |
| Protein Family: | Early growth response protein |
| UniProt Gene Name: | EGR4 |
| UniProt Entry Name: | EGR4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Egr4 antibody (CAB16369) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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