Cell Biology Antibodies 11
Anti-EGR1 Antibody (CAB7266)
- SKU:
- CAB7266
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-EGR1 Antibody |
| Antibody SKU: | CAB7266 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human EGR1 (NP_001955.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:200 - 1:1000 IHC 1:20 - 1:200 IF 1:10 - 1:100 IP 1:20 - 1:50 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-431, OVCAR3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human EGR1 (NP_001955.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAA KAEM QLMS PLQI SDPF GSFP HSPT MDNY PKLE EMML LSNG APQF LGAA GAPE GSGS NSSS SSSG GGGG GGGG SNSS SSSS TFNP QADT GEQP YEHL TAES FPDI SLNN EKVL VETS YPSQ TTRL PPIT YTGR FSLE PAPN SGNT LWPE PLFS LVSG LVSM TNPP ASSS SAPS PAAS SASA SQSP PLSC AVPS NDSS PIYS AAPT FPTP NTDI FPEP QSQA FPGS AGTA LQYP PPAY PAAK GGFQ VP |
| Gene ID: | 1958 |
| Uniprot: | P18146 |
| Cellular Location: | Nucleus |
| Calculated MW: | 57kDa |
| Observed MW: | 51kDa |
| Synonyms: | EGR1, AT225, G0S30, KROX-24, NGFI-A, TIS8, ZIF-268, ZNF225 |
| Background: | The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppressor gene. |
| UniProt Protein Function: | EGR1: Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation. By growth factors. Belongs to the EGR C2H2-type zinc-finger protein family. |
| UniProt Protein Details: | Protein type:DNA-binding; C2H2-type zinc finger protein; Transcription factor Chromosomal Location of Human Ortholog: 5q31.1 Cellular Component: nucleoplasm; cytoplasm; nucleus Molecular Function:histone acetyltransferase binding; protein binding; DNA binding; sequence-specific DNA binding; metal ion binding; double-stranded DNA binding; transcription factor binding; transcription factor activity Biological Process: regulation of long-term neuronal synaptic plasticity; circadian rhythm; response to nutrient levels; transcription from RNA polymerase II promoter; cytokine and chemokine mediated signaling pathway; positive regulation of transcription, DNA-dependent; response to amphetamine; negative regulation of transcription from RNA polymerase II promoter; response to cocaine; BMP signaling pathway; response to ethanol; learning and/or memory; cellular response to insulin stimulus; positive regulation of neuron apoptosis; response to glucose stimulus; oligodendrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; response to electrical stimulus; T cell differentiation; regulation of protein sumoylation; negative regulation of apoptosis |
| NCBI Summary: | The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppressor gene. [provided by RefSeq, Dec 2014] |
| UniProt Code: | P18146 |
| NCBI GenInfo Identifier: | 119242 |
| NCBI Gene ID: | 1958 |
| NCBI Accession: | P18146.1 |
| UniProt Related Accession: | P18146 |
| Molecular Weight: | 543 |
| NCBI Full Name: | Early growth response protein 1 |
| NCBI Synonym Full Names: | early growth response 1 |
| NCBI Official Symbol: | EGR1 |
| NCBI Official Synonym Symbols: | TIS8; AT225; G0S30; NGFI-A; ZNF225; KROX-24; ZIF-268 |
| NCBI Protein Information: | early growth response protein 1; EGR-1; zinc finger protein 225; transcription factor ETR103; transcription factor Zif268; zinc finger protein Krox-24; nerve growth factor-induced protein A |
| UniProt Protein Name: | Early growth response protein 1 |
| UniProt Synonym Protein Names: | AT225; Nerve growth factor-induced protein A; NGFI-A; Transcription factor ETR103; Transcription factor Zif268; Zinc finger protein 225; Zinc finger protein Krox-24 |
| Protein Family: | Early growth response protein |
| UniProt Gene Name: | EGR1 |
| UniProt Entry Name: | EGR1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EGR1 antibody (CAB7266) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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