Cell Biology Antibodies 4

Anti-EGF Antibody (CAB13615)

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SKU:
CAB13615
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-EGF Antibody
Antibody SKU:CAB13615
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 850-950 of human EGF (NP_001954.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, NCI-H460, COS7
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 850-950 of human EGF (NP_001954.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GEDA TCQC LKGF AGDG KLCS DIDE CEMG VPVC PPAS SKCI NTEG GYVC RCSE GYQG DGIH CLDI DECQ LGEH SCGE NASC TNTE GGYT CMCA GRLS EPGL I
Gene ID:1950
Uniprot:P01133
Cellular Location:Membrane, Single-pass type I membrane protein
Calculated MW:129kDa/133kDa
Observed MW:134kDa
Synonyms:EGF, HOMG4, URG
Background:This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
NCBI Summary:This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]
UniProt Code:P01133
NCBI GenInfo Identifier:251757262
NCBI Gene ID:1950
NCBI Accession:P01133.2
UniProt Related Accession:P01133
Molecular Weight:6.2
NCBI Full Name:Pro-epidermal growth factor
NCBI Synonym Full Names:epidermal growth factor
NCBI Official Symbol:EGF  
NCBI Official Synonym Symbols:URG; HOMG4  
NCBI Protein Information:pro-epidermal growth factor
UniProt Protein Name:Pro-epidermal growth factor
UniProt Synonym Protein Names:Urogastrone
Protein Family:Epidermal growth factor
UniProt Gene Name:EGF  
UniProt Entry Name:EGF_HUMAN
Anti-EGF Antibody (CAB13615)Western blot analysis of extracts of various cell lines, using EGF antibody (CAB13615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-EGF Antibody (CAB13615)Immunofluorescence analysis of human skin using EGF Rabbit pAb (CAB13615) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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