Cell Biology Antibodies 3

Anti-EGF Antibody (CAB12916)

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SKU:
CAB12916
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-EGF Antibody
Antibody SKU:CAB12916
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 23-250 of human EGF (NP_001954.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 23-250 of human EGF (NP_001954.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PQHW SCPE GTLA GNGN STCV GPAP FLIF SHGN SIFR IDTE GTNY EQLV VDAG VSVI MDFH YNEK RIYW VDLE RQLL QRVF LNGS RQER VCNI EKNV SGMA INWI NEEV IWSN QQEG IITV TDMK GNNS HILL SALK YPAN VAVD PVER FIFW SSEV AGSL YRAD LDGV GVKA LLET SEKI TAVS LDVL DKRL FWIQ YNRE GSNS LICS CDYD GGSV HISK HPTQ HNLF
Gene ID:1950
Uniprot:P01133
Cellular Location:Membrane, Single-pass type I membrane protein
Calculated MW:129kDa/133kDa
Observed MW:134kDa
Synonyms:EGF, HOMG4, URG
Background:This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
NCBI Summary:This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]
UniProt Code:P01133
NCBI GenInfo Identifier:251757262
NCBI Gene ID:1950
NCBI Accession:P01133.2
UniProt Related Accession:P01133
Molecular Weight:6.2
NCBI Full Name:Pro-epidermal growth factor
NCBI Synonym Full Names:epidermal growth factor
NCBI Official Symbol:EGF  
NCBI Official Synonym Symbols:URG; HOMG4  
NCBI Protein Information:pro-epidermal growth factor
UniProt Protein Name:Pro-epidermal growth factor
UniProt Synonym Protein Names:Urogastrone
Protein Family:Epidermal growth factor
UniProt Gene Name:EGF  
UniProt Entry Name:EGF_HUMAN
Anti-EGF Antibody (CAB12916)Western blot analysis of extracts of 293T cells, using EGF antibody (CAB12916) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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