Name: Anti-EFTUD2 Antibody (CAB7040)
Brand: Epigenetics & Nuclear Signaling Antibodies 4
SKU: CAB7040
Availability: OutOfStock

Antibody Name:	Anti-EFTUD2 Antibody
Antibody SKU:	CAB7040
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human EFTUD2 (NP_004238.3).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:20 - 1:50
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, HepG2, HeLa, Mouse testis, Mouse brain, Mouse spleen, Mouse thymus, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human EFTUD2 (NP_004238.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDTD LYDE FGNY IGPE LDSD EDDD ELGR ETKD LDEM DDDD DDDD VGDH DDDH PGME VVLH EDKK YYPT AEEV YGPE VETI VQEE DTQP LTEP IIKP VKTK KFTL MEQT LPVT VYEM DFLA DLMD NSEL IRNV TLCG HLHH GKTC FVDC LIEQ THPE IRKR YDQD LCYT DILF TEQE RGVG IKST PVTV VLPD TKGK SYLF NIMD T
Gene ID:	9343
Uniprot:	Q15029
Cellular Location:	Nucleus
Calculated MW:	105kDa/108kDa/109kDa
Observed MW:	120kDa

Synonyms:	EFTUD2, MFDGA, MFDM, SNRNP116, Snrp116, Snu114, U5-116KD
Background:	This gene encodes a GTPase which is a component of the spliceosome complex which processes precursor mRNAs to produce mature mRNAs. Mutations in this gene are associated with mandibulofacial dysostosis with microcephaly. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	EFTUD2: Component of the U5 snRNP complex required for pre-mRNA splicing. Binds GTP. Defects in EFTUD2 are the cause of mandibulofacial dysostosis with microcephaly (MFDM). A rare syndrome characterized by progressive microcephaly, midface and malar hypoplasia, micrognathia, microtia, dysplastic ears, preauricular skin tags, significant developmental delay, and speech delay. Many patients have major sequelae, including choanal atresia that results in respiratory difficulties, conductive hearing loss, and cleft palate. Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:RNA-binding; Spliceosome; RNA splicing Chromosomal Location of Human Ortholog: 17q21.31 Cellular Component: nucleoplasm; spliceosome; Cajal body; membrane; cytoplasm; nuclear speck Molecular Function:GTPase activity; protein binding; GTP binding Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression; mRNA processing Disease: Mandibulofacial Dysostosis, Guion-almeida Type
NCBI Summary:	This gene encodes a GTPase which is a component of the spliceosome complex which processes precursor mRNAs to produce mature mRNAs. Mutations in this gene are associated with mandibulofacial dysostosis with microcephaly. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
UniProt Code:	Q15029
NCBI GenInfo Identifier:	18202501
NCBI Gene ID:	9343
NCBI Accession:	Q15029.1
UniProt Secondary Accession:	Q15029,Q9BUR0, B4DK30, B4DMC0, D3DX58, K7EJ81,
UniProt Related Accession:	Q15029
Molecular Weight:	972
NCBI Full Name:	116 kDa U5 small nuclear ribonucleoprotein component
NCBI Synonym Full Names:	elongation factor Tu GTP binding domain containing 2
NCBI Official Symbol:	EFTUD2
NCBI Official Synonym Symbols:	MFDM; MFDGA; Snu114; Snrp116; SNRNP116; U5-116KD
NCBI Protein Information:	116 kDa U5 small nuclear ribonucleoprotein component; hSNU114; SNU114 homolog; U5 snRNP-specific protein, 116 kDa; elongation factor Tu GTP-binding domain-containing protein 2
UniProt Protein Name:	116 kDa U5 small nuclear ribonucleoprotein component
UniProt Synonym Protein Names:	Elongation factor Tu GTP-binding domain-containing protein 2; SNU114 homolog; hSNU114; U5 snRNP-specific protein, 116 kDa; U5-116 kDa
Protein Family:	110 kDa U5 small nuclear ribonucleoprotein
UniProt Gene Name:	EFTUD2
UniProt Entry Name:	U5S1_HUMAN

	Western blot analysis of extracts of various cell lines, using EFTUD2 antibody (CAB7040) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 20s.
	Immunohistochemistry of paraffin-embedded rat kidney using EFTUD2 Rabbit pAb (CAB7040) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat stomach using EFTUD2 Rabbit pAb (CAB7040) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human thyroid cancer using EFTUD2 Rabbit pAb (CAB7040) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal cancer using EFTUD2 Rabbit pAb (CAB7040) at dilution of 1:100 (40x lens).