Antibody Name:	Anti-EEF2K Antibody [KO Validated]
Antibody SKU:	CAB5404
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 406-725 of human EEF2K (NP_037434.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HepG2, HeLa, LOVO, 22Rv1, 293T, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 406-725 of human EEF2K (NP_037434.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KLDH LHWP VFSD LDNM ASRD HDHL DNHR ESEN SGDS GYPS EKRG ELDD PEPR EHGH SYSN RKYE SDED SLGS SGRV CVEK WNLL NSSR LHLP RASA VALE VQRL NALD LEKK IGKS ILGK VHLA MVRY HEGG RFCE KGEE WDQE SAVF HLEH AANL GELE AIVG LGLM YSQL PHHI LADV SLKE TEEN KTKG FDYL LKAA EAGD RQSM ILVA RAFD SGQN LSPD RCQD WLEA LHWY NTAL EMTD CDEG GEYD GMQD EPRY MMLA REAE MLFT GGYG LEKD PQRS GDLY TQAA EAAM EAMK GRLA NQYY QKAE EAWA QMEE
Gene ID:	29904
Uniprot:	O00418
Cellular Location:
Calculated MW:	82kDa
Observed MW:	110kDa

Synonyms:

EEF2K, HSU93850, eEF-2K

Background:

This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.

UniProt Protein Function:	EEF2K: an atypical protein kinase of the Alpha family. Dependent on Ca2 ions and calmodulin. May play a key role linking cellular energy status and the inhibition of protein synthesis, a major consumer of metabolic energy. Phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation eEF2K is phosphorylated and activated by AMPK in response to elevated AMP levels, which increase during cellular stress and starvation. Regulated in response to a range of stimuli including TNF-alpha. Overexpressed in breast cancer cell lines and tumors. Activated in rat glioblastoma, and growth was inhibited by the drug Rottlerin. Activity induced in ischemic heart muscle
UniProt Protein Details:	Protein type:Protein kinase, Ser/Thr (non-receptor); Translation; Kinase, protein; Protein kinase, atypical; EC 2.7.11.20; ATYPICAL group; Alpha family; eEF2K subfamily Chromosomal Location of Human Ortholog: 16p12.2 Cellular Component: cytoplasm; cytosol Molecular Function:calmodulin binding; elongation factor-2 kinase activity; translation factor activity, nucleic acid binding; calcium ion binding; ATP binding; protein kinase activity Biological Process: translational elongation; regulation of protein amino acid autophosphorylation; protein amino acid autophosphorylation; insulin receptor signaling pathway
NCBI Summary:	This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008]
UniProt Code:	O00418
NCBI GenInfo Identifier:	254763289
NCBI Gene ID:	29904
NCBI Accession:	O00418.2
UniProt Secondary Accession:	O00418,Q8N588,
UniProt Related Accession:	O00418
Molecular Weight:	725
NCBI Full Name:	Eukaryotic elongation factor 2 kinase
NCBI Synonym Full Names:	eukaryotic elongation factor 2 kinase
NCBI Official Symbol:	EEF2K
NCBI Official Synonym Symbols:	eEF-2K; HSU93850
NCBI Protein Information:	eukaryotic elongation factor 2 kinase; eEF-2 kinase; elongation factor-2 kinase; eukaroytic elongation factor 2 kinase; eukaryotic elongation factor-2 kinase; calmodulin-dependent protein kinase III; calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase
UniProt Protein Name:	Eukaryotic elongation factor 2 kinase
UniProt Synonym Protein Names:	Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase
Protein Family:	Eukaryotic elongation factor 2 kinase
UniProt Gene Name:	EEF2K
UniProt Entry Name:	EF2K_HUMAN

	Western blot analysis of extracts of various cell lines, using EEF2K antibody (CAB5404) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 40s.
	Western blot analysis of extracts from normal (control) and EEF2K knockout (KO) HeLa cells, using EEF2K antibody (CAB5404) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of A-549 cells using EEF2K antibody (CAB5404). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-Phospho-EEF2K-S366 pAb Antibody (CABP0845)	Human Eukaryotic elongation factor 2 kinase (EEF2K) ELISA Kit
Phospho-EEF2K (S366) Antibody (PACO00302)	eEF2K Colorimetric Cell-Based ELISA Kit
Phospho-EEF2K (S359) Antibody (PACO04011)	eEF2K (Phospho-Ser366) Colorimetric Cell-Based ELISA Kit
EEF2K Antibody (PACO04012)
EEF2K Antibody (PACO06449)