Metabolism Antibodies 2
Anti-EEF2 Antibody (CAB2068)
- SKU:
- CAB2068
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-EEF2 Antibody |
| Antibody SKU: | CAB2068 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human EEF2 (NP_001952.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | HeLa, NCI-H460, 293T |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human EEF2 (NP_001952.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MVNF TVDQ IRAI MDKK ANIR NMSV IAHV DHGK STLT DSLV CKAG IIAS ARAG ETRF TDTR KDEQ ERCI TIKS TAIS LFYE LSEN DLNF IKQS KDGA GFLI NLID SPGH VDFS SEVT AALR VTDG ALVV VDCV SGVC VQTE TVLR QAIA ERIK PVLM MNKM DRAL LELQ LEPE ELYQ TFQR IVEN VNVI ISTY GEGE SGPM GNIM IDPV LGTV GFGS GLHG WAFT LKQF AEMY VAKF AAKG EGQL GPAE RAKK VEDM MKKL WGDR YFDP ANGK FSKS ATSP EGKK LPRT FC |
| Gene ID: | 1938 |
| Uniprot: | P13639 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 95kDa |
| Observed MW: | 107kDa |
| Synonyms: | EEF2, EEF-2, EF-2, EF2, SCA26 |
| Background: | This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. |
| UniProt Protein Function: | EEF2: a member of the GTP-binding translation elongation factor family. An essential factor for protein synthesis. Promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by eEF2 kinase phosphorylation. eEF2 kinase is normally dependent on Ca2+ ions and calmodulin. eEF2 kinase can also be activated by PKA in response to elevated cAMP levels, which are generally increased in stress- or starvation-related conditions. A variety of treatments known to raise intracellular Ca2+ or cAMP levels have been shown to result in increased phosphorylation of eEF2, and thus to inhibit peptide-chain elongation. |
| UniProt Protein Details: | Protein type:Translation elongation; Translation Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: polysome; membrane; cytoplasm; plasma membrane; cytosol; ribonucleoprotein complex; nucleus Molecular Function:GTPase activity; protein binding; GTP binding; protein kinase binding; translation elongation factor activity Biological Process: translational elongation; cellular protein metabolic process; translation; positive regulation of translation; peptidyl-diphthamide biosynthetic process from peptidyl-histidine; pathogenesis; hemopoietic progenitor cell differentiation; gene expression; post-translational protein modification Disease: Spinocerebellar Ataxia 26 |
| NCBI Summary: | This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P13639 |
| NCBI GenInfo Identifier: | 119172 |
| NCBI Gene ID: | 1938 |
| NCBI Accession: | P13639.4 |
| UniProt Secondary Accession: | P13639,Q58J86, B2RMP5, D6W618, |
| UniProt Related Accession: | P13639 |
| Molecular Weight: | 858 |
| NCBI Full Name: | Elongation factor 2 |
| NCBI Synonym Full Names: | eukaryotic translation elongation factor 2 |
| NCBI Official Symbol: | EEF2 |
| NCBI Official Synonym Symbols: | EF2; EF-2; EEF-2; SCA26 |
| NCBI Protein Information: | elongation factor 2; polypeptidyl-tRNA translocase |
| UniProt Protein Name: | Elongation factor 2 |
| Protein Family: | Elongation factor |
| UniProt Gene Name: | EEF2 |
| UniProt Entry Name: | EF2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EEF2 antibody (CAB2068) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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