Name: Anti-EEF1G Antibody (CAB7891)
Brand: Metabolism Antibodies 2
SKU: CAB7891
Availability: OutOfStock

Antibody Name:	Anti-EEF1G Antibody
Antibody SKU:	CAB7891
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 168-437 of human EEF1G (NP_001395.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	BxPC-3, NIH/3T3, HeLa, Mouse brain, Mouse thymus, Mouse pancreas, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 168-437 of human EEF1G (NP_001395.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LLWL YKQV LEPS FRQA FPNT NRWF LTCI NQPQ FRAV LGEV KLCE KMAQ FDAK KFAE TQPK KDTP RKEK GSRE EKQK PQAE RKEE KKAA APAP EEEM DECE QALA AEPK AKDP FAHL PKST FVLD EFKR KYSN EDTL SVAL PYFW EHFD KDGW SLWY SEYR FPEE LTQT FMSC NLIT GMFQ RLDK LRKN AFAS VILF GTNN SSSI SGVW VFRG QELA FPLS PDWQ VDYE SYTW RKLD PGSE ETQT LVRE YFSW EGAF QHVG KAFN QGKI FK
Gene ID:	1937
Uniprot:	P26641
Cellular Location:
Calculated MW:	50kDa/56kDa
Observed MW:	50kDa

Synonyms:	EEF1G, EF1G, GIG35
Background:	This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases.

UniProt Protein Function:	EEF1G: Probably plays a role in anchoring the complex to other cellular components.
UniProt Protein Details:	Protein type:Translation; Translation elongation Chromosomal Location of Human Ortholog: 11q12.3 Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; membrane; nucleus Molecular Function:cadherin binding; protein binding Biological Process: response to virus; translational elongation
NCBI Summary:	This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases. [provided by RefSeq, Jul 2008]
UniProt Code:	P26641
NCBI GenInfo Identifier:	119165
NCBI Gene ID:	1937
NCBI Accession:	P26641.3
UniProt Secondary Accession:	P26641,Q6PJ62, Q6PK31, Q96CU2, Q9P196, B4DTG2,
UniProt Related Accession:	P26641
Molecular Weight:	50kDa
NCBI Full Name:	Elongation factor 1-gamma
NCBI Synonym Full Names:	eukaryotic translation elongation factor 1 gamma
NCBI Official Symbol:	EEF1G
NCBI Official Synonym Symbols:	EF1G; GIG35
NCBI Protein Information:	elongation factor 1-gamma
UniProt Protein Name:	Elongation factor 1-gamma
UniProt Synonym Protein Names:	eEF-1B gamma
Protein Family:	Elongation factor
UniProt Gene Name:	EEF1G

	Western blot analysis of extracts of various cell lines, using EEF1G antibody (CAB7891) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded rat brain using EEF1G Rabbit pAb (CAB7891) at dilution of 1:50 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using EEF1G Rabbit pAb (CAB7891) at dilution of 1:50 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using EEF1G Rabbit pAb (CAB7891) at dilution of 1:50 (40x lens).
	Immunofluorescence analysis of C6 cells using EEF1G Polyclonal Antibody (CAB7891) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.