Antibody Name:	Anti-EEF1E1 Antibody
Antibody SKU:	CAB15357
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EEF1E1 (NP_004271.1).

Application:	WB IF
Recommended Dilution:	WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, HeLa, Mouse brain, Rat brain

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EEF1E1 (NP_004271.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAA AELS LLEK SLGL SKGN KYSA QGER QIPV LQTN NGPS LTGL TTIA AHLV KQAN KEYL LGST AEEK AIVQ QWLE YRVT QVDG HSSK NDIH TLLK DLNS
Gene ID:	9521
Uniprot:	O43324
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	15kDa/19kDa
Observed MW:	20kDa

Synonyms:

EEF1E1, AIMP3, P18

Background:

This gene encodes a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage, and it plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream MUTED (muted homolog) gene. An EEF1E1-related pseudogene has been identified on chromosome 2.

UniProt Protein Function:	EEF1E1: Positive modulator of ATM response to DNA damage.
UniProt Protein Details:	Protein type:Translation; Translation elongation Chromosomal Location of Human Ortholog: 6p24.3 Cellular Component: cytoplasm; nucleus; cytosol Molecular Function:protein binding Biological Process: negative regulation of cell proliferation; tRNA aminoacylation for protein translation; positive regulation of apoptosis; gene expression; positive regulation of DNA damage response, signal transduction by p53 class mediator
NCBI Summary:	This gene encodes a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage, and it plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream MUTED (muted homolog) gene. An EEF1E1-related pseudogene has been identified on chromosome 2. [provided by RefSeq, Dec 2010]
UniProt Code:	O43324
NCBI GenInfo Identifier:	17378996
NCBI Gene ID:	9521
NCBI Accession:	O43324.1
UniProt Related Accession:	O43324
Molecular Weight:	20; 16; 20kDa
NCBI Full Name:	Eukaryotic translation elongation factor 1 epsilon-1
NCBI Synonym Full Names:	eukaryotic translation elongation factor 1 epsilon 1
NCBI Official Symbol:	EEF1E1
NCBI Official Synonym Symbols:	P18; AIMP3
NCBI Protein Information:	eukaryotic translation elongation factor 1 epsilon-1
UniProt Protein Name:	Eukaryotic translation elongation factor 1 epsilon-1
UniProt Synonym Protein Names:	Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3; Elongation factor p18; Multisynthase complex auxiliary component p18
Protein Family:	Eukaryotic translation elongation factor
UniProt Gene Name:	EEF1E1
UniProt Entry Name:	MCA3_HUMAN

	Western blot analysis of extracts of various cell lines, using EEF1E1 antibody (CAB15357) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of L929 cells using EEF1E1 antibody (CAB15357) at dilution of 1:100. Blue: DAPI for nuclear staining.