KO Validated Antibodies 1

Anti-EEF1B2 Antibody (CAB6580)[KO Validated]

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SKU:
CAB6580
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-EEF1B2 Antibody [KO Validated]
Antibody SKU:CAB6580
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human EEF1B2 (NP_001032752.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:MCF-7, HL-60, HeLa, 293T, Mouse pancreas
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human EEF1B2 (NP_001032752.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGFG DLKS PAGL QVLN DYLA DKSY IEGY VPSQ ADVA VFEA VSSP PPAD LCHA LRWY NHIK SYEK EKAS LPGV KKAL GKYG PADV EDTT GSGA TDSK DDDD IDLF GSDD EEES EEAK RLRE ERLA QYES KKAK KPAL VAKS SILL DVKP WDDE TDMA KLEE CVRS IQAD GLVW GSSK LVPV GYGI KKLQ IQCV VEDD KVGT DMLE EQIT AFED YVQS MDVA AFNK I
Gene ID:1933
Uniprot:P24534
Cellular Location:
Calculated MW:24kDa
Observed MW:33kDa
Synonyms:EEF1B2, EEF1B, EEF1B1, EF1B
Background:This gene encodes a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. Alternative splicing results in three transcript variants which differ only in the 5' UTR.
UniProt Protein Function:EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP.
NCBI Summary:This gene encodes a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. Alternative splicing results in three transcript variants which differ only in the 5' UTR. [provided by RefSeq, Jul 2008]
UniProt Code:P24534
NCBI GenInfo Identifier:119163
NCBI Gene ID:1933
NCBI Accession:P24534.3
UniProt Secondary Accession:P24534,Q6IBH9, A8K795,
UniProt Related Accession:P24534
Molecular Weight:24,764 Da
NCBI Full Name:Elongation factor 1-beta
NCBI Synonym Full Names:eukaryotic translation elongation factor 1 beta 2
NCBI Official Symbol:EEF1B2  
NCBI Official Synonym Symbols:EF1B; EEF1B; EEF1B1  
NCBI Protein Information:elongation factor 1-beta
UniProt Protein Name:Elongation factor 1-beta
UniProt Gene Name:EEF1B2  
UniProt Entry Name:EF1B_HUMAN
Anti-EEF1B2 Antibody [KO Validated] (CAB6580)Western blot analysis of extracts of various cell lines, using EEF1B2 antibody (CAB6580) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-EEF1B2 Antibody [KO Validated] (CAB6580)Western blot analysis of extracts from normal (control) and EEF1B2 knockout (KO) 293T cells, using EEF1B2 antibody (CAB6580) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-EEF1B2 Antibody [KO Validated] (CAB6580)Immunohistochemistry of paraffin-embedded rat spinal cord using EEF1B2 Antibody (CAB6580) at dilution of 1:100 (40x lens).
Anti-EEF1B2 Antibody [KO Validated] (CAB6580)Immunohistochemistry of paraffin-embedded rat brain using EEF1B2 Antibody (CAB6580) at dilution of 1:100 (40x lens).
Anti-EEF1B2 Antibody [KO Validated] (CAB6580)Immunohistochemistry of paraffin-embedded mouse brain using EEF1B2 Antibody (CAB6580) at dilution of 1:100 (40x lens).
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