Immunology Antibodies 2
Anti-EED Antibody (CAB5371)
- SKU:
- CAB5371
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-EED Antibody |
| Antibody SKU: | CAB5371 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human EED (NP_694536.1). |
| Application: | WB IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, 293T, HepG2, A-549, SKOV3, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human EED (NP_694536.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSER EVST APAG TDMP AAKK QKLS SDEN SNPD LSGD ENDD AVSI ESGT NTER PDTP TNTP NAPG RKSW GKGK WKSK KCKY SFKC VNSL KEDH NQPL FGVQ FNWH SKEG DPLV FATV GSNR VTLY ECHS QGEI RLLQ SYVD ADAD ENFY TCAW TYDS NTSH PLLA VAGS RGII RIIN PITM QCIK HYVG HGNA INEL KFHP RDPN LLLS VSKD HALR LWNI QTDT LVAI FGGV EGHR DEVL SADY DLLG EKIM SCGM DHSL KLWR INSK RMMN AIKE SYDY NPNK TNRP FISQ KIHF PDFS |
| Gene ID: | 8726 |
| Uniprot: | O75530 |
| Cellular Location: | Chromosome, Nucleus |
| Calculated MW: | 45kDa/50kDa/53kDa |
| Observed MW: | 50-80kDa |
| Synonyms: | EED, HEED, WAIT1 |
| Background: | This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene. |
| UniProt Protein Function: | Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. |
| NCBI Summary: | This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O75530 |
| NCBI GenInfo Identifier: | 205790383 |
| NCBI Gene ID: | 8726 |
| NCBI Accession: | O75530.2 |
| UniProt Secondary Accession: | O75530,O00149, Q6NTH2, Q7LDA5, Q7LDG8, Q86VV2, Q9UNY7 A8K7V5, |
| UniProt Related Accession: | O75530 |
| Molecular Weight: | 50kDa |
| NCBI Full Name: | Polycomb protein EED |
| NCBI Synonym Full Names: | embryonic ectoderm development |
| NCBI Official Symbol: | EED |
| NCBI Official Synonym Symbols: | HEED; WAIT1 |
| NCBI Protein Information: | polycomb protein EED |
| UniProt Protein Name: | Polycomb protein EED |
| UniProt Synonym Protein Names: | Embryonic ectoderm development protein |
| Protein Family: | Polycomb protein |
| UniProt Gene Name: | EED |
 | Western blot analysis of extracts of various cell lines, using EED antibody (CAB5371) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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