KO Validated Antibodies 1

Anti-EDF1 Antibody (CAB2283)[KO Validated]

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SKU:
CAB2283
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Developmental Biology

Description

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Antibody Name:Anti-EDF1 Antibody [KO Validated]
Antibody SKU:CAB2283
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-148 of human EDF1 (NP_003783.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, Mouse brain, Mouse liver, Rat brain, NIH/3T3
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-148 of human EDF1 (NP_003783.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAES DWDT VTVL RKKG PTAA QAKS KQAI LAAQ RRGE DVET SKKW AAGQ NKQH SITK NTAK LDRE TEEL HHDR VTLE VGKV IQQG RQSK GLTQ KDLA TKIN EKPQ VIAD YESG RAIP NNQV LGKI ERAI GLKL RGKD IGKP IEKG PRAK
Gene ID:8721
Uniprot:O60869
Cellular Location:Cytoplasm, Nucleus
Calculated MW:15kDa/16kDa
Observed MW:16kDa
Synonyms:EDF1, CFAP280, EDF-1, MBF1
Background:This gene encodes a protein that may regulate endothelial cell differentiation, lipid metabolism, and hormone-induced cardiomyocyte hypertrophy. The encoded protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Alternate splicing results in multiple transcript variants.
UniProt Protein Function:EDF1: Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription, coactivator/corepressor; DNA-binding; Nuclear receptor co-regulator; RNA-binding

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: transcription factor TFIID complex; cytoplasm; nucleolus; intracellular; nucleus

Molecular Function:calmodulin binding; methyltransferase activity; protein binding; histone acetyltransferase activity; sequence-specific DNA binding; transcription coactivator activity; transcription factor activity

Biological Process: endothelial cell differentiation; regulation of lipid metabolic process; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; multicellular organismal development; positive regulation of DNA binding

UniProt Code:O60869
NCBI GenInfo Identifier:62510569
NCBI Gene ID:
NCBI Accession:O60869.1
Molecular Weight:~ 16kDa
NCBI Full Name:Endothelial differentiation-related factor 1
UniProt Protein Name:Endothelial differentiation-related factor 1
UniProt Synonym Protein Names:Multiprotein-bridging factor 1; MBF1
Protein Family:Endothelial differentiation-related factor
UniProt Gene Name:EDF1  
UniProt Entry Name:EDF1_HUMAN
Anti-EDF1 Antibody [KO Validated] (CAB2283)Western blot analysis of extracts of various cell lines, using EDF1 antibody (CAB2283) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-EDF1 Antibody [KO Validated] (CAB2283)Western blot analysis of extracts from normal (control) and EDF1 Rabbit pAb knockout (KO) NIH/3T3 cells, using EDF1 Rabbit pAb antibody (CAB2283) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-EDF1 Antibody [KO Validated] (CAB2283)Immunohistochemistry of paraffin-embedded rat brain using EDF1 antibody (CAB2283) at dilution of 1:100 (40x lens).
Anti-EDF1 Antibody [KO Validated] (CAB2283)Immunohistochemistry of paraffin-embedded human gastric cancer using EDF1 antibody (CAB2283) at dilution of 1:100 (40x lens).
Anti-EDF1 Antibody [KO Validated] (CAB2283)Immunohistochemistry of paraffin-embedded mouse lung using EDF1 antibody (CAB2283) at dilution of 1:100 (40x lens).
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