Cell Biology Antibodies 6

Anti-EDC4 Antibody (CAB15418)

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SKU:
CAB15418
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-EDC4 Antibody
Antibody SKU:CAB15418
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 800-1100 of human EDC4 (NP_055144.3).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human, Mouse
Positive Samples:Jurkat, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 800-1100 of human EDC4 (NP_055144.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GDGD RHNT PSLL EAAL TQEA STPD SQVW PTAP DITR ETCS TLAE SPRN GLQE KHKS LAFH RPPY HLLQ QRDS QDAS AEQS DHDD EVAS LASA SGGF GTKV PAPR LPAK DWKT KGSP RTSP KLKR KSKK DDGD AAMG SRLT EHQV AEPP EDWP ALIW QQQR ELAE LRHS QEEL LQRL CTQL EGLQ STVT GHVE RALE TRHE QEQR RLER ALAE GQQR GGQL QEQL TQQL SQAL SSAV AGRL ERSI RDEI KKTV PPCV SRSL EPMA GQLS NSVA TKLT AVEG SMKE NISK LLKS KNLT DAIA R
Gene ID:23644
Uniprot:Q6P2E9
Cellular Location:Cytoplasm, Nucleus, P-body
Calculated MW:109kDa/151kDa
Observed MW:160-180kDa
Synonyms:EDC4, GE1, Ge-1, HEDL5, HEDLS, RCD-8, RCD8
Background:
UniProt Protein Function:EDC4: a protein required for the 5' a?? 3' pathway of mRNA decapping and degradation-based post-transcriptional gene silencing. The proteins involved in 5' a?? 3' mRNA decay include DCP2, DCP1A, EDC3, EDC4 and DDX6, and are concentrated in cytoplasmic structures called mRNA "processing bodies" (P-bodies). Shortening of the 3'-poly(A) tail and removal of PABP precedes cleavage of the 5'-mRNA cap by a complex containing decapping enzymes 1A and 2 (DCP1A/DCP2). EDC4 enhances the catalytic activity of DCP2 in vitro. Shown to be required for silencing by miRNAs in Drosophila melanogaster. Two alternatively spliced human isoforms have been described.
UniProt Protein Details:

Protein type:RNA processing

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: nucleoplasm; membrane; intracellular membrane-bound organelle; cytoplasm; nucleus; cytosol

Molecular Function:protein binding

Biological Process: gene expression; mRNA catabolic process, deadenylation-dependent decay

UniProt Code:Q6P2E9
NCBI GenInfo Identifier:74758241
NCBI Gene ID:23644
NCBI Accession:Q6P2E9.1
UniProt Secondary Accession:Q6P2E9,Q13025, Q13826, Q6ZR12, Q7Z6H7, A6NGM1, A8K4T4
UniProt Related Accession:Q6P2E9
Molecular Weight:151,661 Da
NCBI Full Name:Enhancer of mRNA-decapping protein 4
NCBI Synonym Full Names:enhancer of mRNA decapping 4
NCBI Official Symbol:EDC4  
NCBI Official Synonym Symbols:GE1; Ge-1; RCD8; HEDL5; HEDLS; RCD-8  
NCBI Protein Information:enhancer of mRNA-decapping protein 4; autoantigen Ge-1; autoantigen RCD-8; human enhancer of decapping large subunit
UniProt Protein Name:Enhancer of mRNA-decapping protein 4
UniProt Synonym Protein Names:Autoantigen Ge-1; Autoantigen RCD-8; Human enhancer of decapping large subunit
Protein Family:Enhancer of mRNA-decapping protein
UniProt Gene Name:EDC4  
UniProt Entry Name:EDC4_HUMAN
Anti-EDC4 Antibody (CAB15418)Western blot analysis of extracts of various cell lines, using EDC4 antibody (CAB15418) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
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