Immunology Antibodies 2

Anti-ECSIT Antibody (CAB7804)

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SKU:
CAB7804
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-ECSIT Antibody
Antibody SKU:CAB7804
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 50-296 of human ECSIT (NP_001135936.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:HepG2, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 50-296 of human ECSIT (NP_001135936.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SEQS LVPS PPEP RQRP TKAL VPFE DLFG QAPG GERD KASF LQTV QKFA EHSV RKRG HIDF IYLA LRKM REYG VERD LAVY NQLL NIFP KEVF RPRN IIQR IFVH YPRQ QECG IAVL EQME NHGV MPNK ETEF LLIQ IFGR KSYP MLKL VRLK LWFP RFMN VNPF PVPR DLPQ DPVE LAMF GLRH MEPD LSAR VTIY QVPL PKDS TGAA DPPQ PHIV GSGR DAGG VEPL LPDA AGPG VCEE WLGQ LRV
Gene ID:51295
Uniprot:Q9BQ95
Cellular Location:Cytoplasm, Mitochondrion, Nucleus
Calculated MW:24kDa/33kDa/49kDa
Observed MW:49kDa
Synonyms:ECSIT, SITPEC
Background:
UniProt Protein Function:ECSIT: Adapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development. Required for efficient assembly of mitochondrial NADH:ubiquinone oxidoreductase. Belongs to the ECSIT family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription factor; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: cytoplasm; mitochondrial inner membrane; mitochondrion; nucleoplasm; nucleus

Molecular Function:oxidoreductase activity, acting on NADH or NADPH; protein binding

Biological Process: mitochondrial respiratory chain complex I assembly; regulation of oxidoreductase activity

UniProt Code:Q9BQ95
NCBI GenInfo Identifier:74752234
NCBI Gene ID:51295
NCBI Accession:Q9BQ95.1
UniProt Secondary Accession:Q9BQ95,Q96HQ7, Q9NYI1, E9PAN9, K7EMM0,
UniProt Related Accession:Q9BQ95
Molecular Weight:33,938 Da
NCBI Full Name:Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial
NCBI Synonym Full Names:ECSIT signalling integrator
NCBI Official Symbol:ECSIT  
NCBI Official Synonym Symbols:SITPEC  
NCBI Protein Information:evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial
UniProt Protein Name:Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial
UniProt Synonym Protein Names:Protein SITPEC
Protein Family:Evolutionarily conserved signaling intermediate
UniProt Gene Name:ECSIT  
UniProt Entry Name:ECSIT_HUMAN
Anti-ECSIT Antibody (CAB7804)Western blot analysis of extracts of various cell lines, using ECSIT antibody (CAB7804) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-ECSIT Antibody (CAB7804)Immunohistochemistry of paraffin-embedded human gastric cancer using ECSIT antibody (CAB7804) at dilution of 1:100 (40x lens).
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