Antibody Name:	Anti-ECI2 Antibody
Antibody SKU:	CAB13072
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-364 of human ECI2 (NP_006108.2).

Application:	WB IHC
Recommended Dilution:	WB 1:1000 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Mouse kidney, Rat liver, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-364 of human ECI2 (NP_006108.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MNRT AMRA SQKD FENS MNQV KLLK KDPG NEVK LKLY ALYK QATE GPCN MPKP GVFD LINK AKWD AWNA LGSL PKEA ARQN YVDL VSSL SPSL ESSS QVEP GTDR KSTG FETL VVTS EDGI TKIM FNRP KKKN AINT EMYH EIMR ALKA ASKD DSII TVLT GNGD YYSS GNDL TNFT DIPP GGVE EKAK NNAV LLRE FVGC FIDF PKPL IAVV NGPA VGIS VTLL GLFD AVYA SDRA TFHT PFSH LGQS PEGC SSYT FPKI MSPA KATE MLIF GKKL TAGE ACAQ GLVT EVFP DSTF QKEV WTRL KAFA KLPP NALR ISKE VIRK RERE KLHA VNAE ECNV LQGR WLSD ECTN AVVN FLSR KSKL
Gene ID:	10455
Uniprot:	O75521
Cellular Location:	Mitochondrion, Peroxisome matrix
Calculated MW:	39kDa/43kDa
Observed MW:	55kDa

Synonyms:

ECI2, ACBD2, DRS-1, DRS1, HCA88, PECI, dJ1013A10.3

Background:

This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described.

UniProt Protein Function:	PECI: Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Has a preference for 3-trans substrates. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Isomerase; EC 5.3.3.8; Lipid Metabolism - fatty acid Chromosomal Location of Human Ortholog: 6p24.3 Cellular Component: intracellular membrane-bound organelle; membrane; mitochondrion; nucleoplasm; peroxisomal matrix Molecular Function:dodecenoyl-CoA delta-isomerase activity; receptor binding Biological Process: cellular lipid metabolic process
NCBI Summary:	This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. [provided by RefSeq, Aug 2011]
UniProt Code:	O75521
NCBI GenInfo Identifier:	257051079
NCBI Gene ID:	10455
NCBI Accession:	O75521.4
UniProt Secondary Accession:	O75521,Q5JYK5, Q5JYK7, Q7L124, Q8N0X0, Q9BUE9, Q9H0T9 Q9NQH1, Q9NYH7, Q9UN55,
UniProt Related Accession:	O75521
Molecular Weight:	39,609 Da
NCBI Full Name:	Enoyl-CoA delta isomerase 2, mitochondrial
NCBI Synonym Full Names:	enoyl-CoA delta isomerase 2
NCBI Official Symbol:	ECI2
NCBI Official Synonym Symbols:	DRS1; PECI; ACBD2; DRS-1; HCA88; dJ1013A10.3
NCBI Protein Information:	enoyl-CoA delta isomerase 2, mitochondrial
UniProt Protein Name:	Enoyl-CoA delta isomerase 2, mitochondrial
UniProt Synonym Protein Names:	DRS-1; Delta(3),delta(2)-enoyl-CoA isomerase; D3,D2-enoyl-CoA isomerase; Diazepam-binding inhibitor-related protein 1; DBI-related protein 1; Dodecenoyl-CoA isomerase; Hepatocellular carcinoma-associated antigen 88; Peroxisomal 3,2-trans-enoyl-CoA isomerase; pECI; Renal carcinoma antigen NY-REN-1
Protein Family:	Enoyl-CoA delta isomerase
UniProt Gene Name:	ECI2
UniProt Entry Name:	ECI2_HUMAN

	Western blot analysis of extracts of various cell lines, using ECI2 Rabbit pAb (CAB13072) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human liver cancer using ECI2 antibody (CAB13072) at dilution of 1:150 (40x lens).