Antibody Name:	Anti-ECHS1 Antibody
Antibody SKU:	CAB3845
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 28-290 of human ECHS1 (NP_004083.3).

Application:	WB IF
Recommended Dilution:	WB 1:1000 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	LO2, 293T, U-87MG, MCF7, 22Rv1, Mouse liver, Mouse kidney, Mouse heart, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 28-290 of human ECHS1 (NP_004083.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ASGA NFEY IIAE KRGK NNTV GLIQ LNRP KALN ALCD GLID ELNQ ALKT FEED PAVG AIVL TGGD KAFA AGAD IKEM QNLS FQDC YSSK FLKH WDHL TQVK KPVI AAVN GYAF GGGC ELAM MCDI IYAG EKAQ FAQP EILI GTIP GAGG TQRL TRAV GKSL AMEM VLTG DRIS AQDA KQAG LVSK ICPV ETLV EEAI QCAE KIAS NSKI VVAM AKES VNAA FEMT LTEG SKLE KKLF YSTF ATDD RKEG MTAF VEKR KANF KDQ
Gene ID:	1892
Uniprot:	P30084
Cellular Location:	Mitochondrion matrix
Calculated MW:	31kDa
Observed MW:	31kDa

Synonyms:

ECHS1, ECHS1D, SCEH

Background:

The protein encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature.

UniProt Protein Function:	Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate. Has high substrate specificity for crotonyl-CoA and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA and methacrylyl-CoA. It is noteworthy that binds tiglyl-CoA, but hydrates only a small amount of this substrate.
NCBI Summary:	The protein encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. [provided by RefSeq, Jul 2008]
UniProt Code:	P30084
NCBI GenInfo Identifier:	62906863
NCBI Gene ID:	1892
NCBI Accession:	P30084.4
UniProt Secondary Accession:	P30084,O00739, Q5VWY1, Q96H54,
UniProt Related Accession:	P30084
Molecular Weight:	31,387 Da
NCBI Full Name:	Enoyl-CoA hydratase, mitochondrial
NCBI Synonym Full Names:	enoyl-CoA hydratase, short chain 1
NCBI Official Symbol:	ECHS1
NCBI Official Synonym Symbols:	SCEH; ECHS1D
NCBI Protein Information:	enoyl-CoA hydratase, mitochondrial
UniProt Protein Name:	Enoyl-CoA hydratase, mitochondrial
UniProt Synonym Protein Names:	Enoyl-CoA hydratase 1; Short-chain enoyl-CoA hydratase; SCEH
Protein Family:	Enoyl-CoA hydratase
UniProt Gene Name:	ECHS1

	Western blot analysis of extracts of various cell lines, using ECHS1 antibody (CAB3845) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of HeLa cells using ECHS1 antibody (CAB3845) at dilution of 1:100. Blue: DAPI for nuclear staining.