Name: Anti-ECH1 Antibody (CAB19868)[KO Validated]
Brand: KO Validated Antibodies 2
SKU: CAB19868
Availability: OutOfStock

Antibody Name:	Anti-ECH1 Antibody [KO Validated]
Antibody SKU:	CAB19868
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human ECH1.

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T

Immunogen:	Recombinant protein of human ECH1.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	1891
Uniprot:	Q13011
Cellular Location:	Mitochondrion, Peroxisome
Calculated MW:	35kDa
Observed MW:	32KDa

Anti-ECH1 Antibody [KO Validated] (CAB19868)

Western blot analysis of extracts from normal (control) and ECH1 knockout (KO) 293T cells, using ECH1 antibody (CAB19868) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Synonyms:

ECH1, HPXEL

Background:

This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans, 5-cis-dienoyl-CoA to 2-trans, 4-trans-dienoyl-CoA, indicating that it is a delta3, 5-delta2, 4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.

UniProt Protein Function:	ECH1: Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4- trans-dienoyl-CoA. Belongs to the enoyl-CoA hydratase/isomerase family.
UniProt Protein Details:	Protein type:Mitochondrial; Isomerase; Amino Acid Metabolism - tyrosine; EC 5.3.3.- Chromosomal Location of Human Ortholog: 19q13.1 Cellular Component: membrane Molecular Function:protein binding; receptor binding
NCBI Summary:	This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13011
NCBI GenInfo Identifier:	82654933
NCBI Gene ID:	1891
NCBI Accession:	Q13011.2
UniProt Secondary Accession:	Q13011,Q8WVX0, Q96EZ9, A8K745,
UniProt Related Accession:	Q13011
Molecular Weight:	35,816 Da
NCBI Full Name:	Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
NCBI Synonym Full Names:	enoyl-CoA hydratase 1
NCBI Official Symbol:	ECH1
NCBI Official Synonym Symbols:	HPXEL
NCBI Protein Information:	delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
UniProt Protein Name:	Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
UniProt Gene Name:	ECH1
UniProt Entry Name:	ECH1_HUMAN

Antibodies	ELISA Kits
Anti-ECH1 Antibody (CAB12944)	Human Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial (ECH1) ELISA Kit
ECH1 Antibody (PACO08985)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)