Metabolism Antibodies 1

Anti-ECH1 Antibody (CAB12944)

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SKU:
CAB12944
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-ECH1 Antibody
Antibody SKU:CAB12944
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human ECH1 (NP_001389.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:A375, BxPC-3, HepG2, Jurkat, MCF7, K-562, Mouse testis, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human ECH1 (NP_001389.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAAG IVAS RRLR DLLT RRLT GSNY PGLS ISLR LTGS SAQE EASG VALG EAPD HSYE SLRV TSAQ KHVL HVQL NRPN KRNA MNKV FWRE MVEC FNKI SRDA DCRA VVIS GAGK MFTA GIDL MDMA SDIL QPKG DDVA RISW YLRD IITR YQET FNVI ERCP KPVI AAVH GGCI GGGV DLVT ACDI RYCA QDAF FQVK EVDV GLAA DVGT LQRL PKVI GNQS LVNE LAFT ARKM MADE ALGS GLVS RVFP DKEV MLDA ALAL AAEI SSKS PVAV QSTK VNLL YSRD HSVA ESLN YVAS WNMS MLQT QDLV KSVQ ATTE NKEL KTVT FSKL
Gene ID:1891
Uniprot:Q13011
Cellular Location:Mitochondrion, Peroxisome
Calculated MW:35kDa
Observed MW:36kDa
Synonyms:ECH1, HPXEL
Background:This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans, 5-cis-dienoyl-CoA to 2-trans, 4-trans-dienoyl-CoA, indicating that it is a delta3, 5-delta2, 4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.
UniProt Protein Function:ECH1: Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4- trans-dienoyl-CoA. Belongs to the enoyl-CoA hydratase/isomerase family.
UniProt Protein Details:

Protein type:Mitochondrial; Isomerase; Amino Acid Metabolism - tyrosine; EC 5.3.3.-

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: membrane

Molecular Function:protein binding; receptor binding

NCBI Summary:This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq, Jul 2008]
UniProt Code:Q13011
NCBI GenInfo Identifier:82654933
NCBI Gene ID:1891
NCBI Accession:Q13011.2
UniProt Secondary Accession:Q13011,Q8WVX0, Q96EZ9, A8K745,
UniProt Related Accession:Q13011
Molecular Weight:35,816 Da
NCBI Full Name:Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
NCBI Synonym Full Names:enoyl-CoA hydratase 1
NCBI Official Symbol:ECH1  
NCBI Official Synonym Symbols:HPXEL  
NCBI Protein Information:delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
UniProt Protein Name:Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
UniProt Gene Name:ECH1  
UniProt Entry Name:ECH1_HUMAN
Anti-ECH1 Antibody (CAB12944)Western blot analysis of extracts of various cell lines, using ECH1 Antibody (CAB12944) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-ECH1 Antibody (CAB12944)Immunohistochemistry of paraffin-embedded human placenta using ECH1 antibody (CAB12944) at dilution of 1:150 (40x lens).
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