Cell Biology Antibodies 10
Anti-ECE2 Antibody (CAB7049)
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- SKU:
- CAB7049
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-ECE2 Antibody |
| Antibody SKU: | CAB7049 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human ECE2 (NP_115707.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | BT-474, HepG2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human ECE2 (NP_115707.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MASP GAGR APPE LPER NCGY REVE YWDQ RYQG AADS APYD WFGD FSSF RALL EPEL RPED RILV LGCG NSAL SYEL FLGG FPNV TSVD YSSV VVAA MQAR HAHV PQLR WETM DVRK LDFP SASF DVVL EKGT LDAL LAGE RDPW TVSS EGVH TVDQ VLSE |
| Gene ID: | 9718 |
| Uniprot: | O60344 |
| Cellular Location: | Cytoplasmic granule membrane, Golgi apparatus membrane, Single-pass type II membrane protein |
| Calculated MW: | 28kDa/83kDa/86kDa/91kDa/99kDa |
| Observed MW: | 90kDa |
| Synonyms: | ECE2 |
| Background: | This gene encodes a member of the M13 family, which includes type 2 integral membrane metallopeptidases. The encoded enzyme is a membrane-bound zinc-dependent metalloprotease. The enzyme catalyzes the cleavage of big endothelin to produce the vasoconstrictor endothelin-1, and plays a role in the processing of several neuroendocrine peptides. It may also have methyltransferase activity. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | ECE2: Converts big endothelin-1 to endothelin-1. Also involved in the processing of various neuroendocrine peptides, including neurotensin, angiotensin I, substance P, proenkephalin-derived peptides, and prodynorphin-derived peptides. May limit beta- amyloid peptide accumulation in brain. May also have methyltransferase activity. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 3.4.24.71; Methyltransferase, protein arginine, predicted; Vesicle; Methyltransferase; Protease; Membrane protein, integral Chromosomal Location of Human Ortholog: 3q27.1 Cellular Component: cytoplasmic vesicle membrane Molecular Function:metalloendopeptidase activity; protein binding Biological Process: brain development; cardioblast differentiation; cell-cell signaling; heart development; peptide hormone processing |
| NCBI Summary: | This gene encodes a member of the M13 family, which includes type 2 integral membrane metallopeptidases. The encoded enzyme is a membrane-bound zinc-dependent metalloprotease. The enzyme catalyzes the cleavage of big endothelin to produce the vasoconstrictor endothelin-1, and plays a role in the processing of several neuroendocrine peptides. It may also have methyltransferase activity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] |
| UniProt Code: | O60344 |
| NCBI GenInfo Identifier: | 215274248 |
| NCBI Gene ID: | 9718 |
| NCBI Accession: | O60344.4 |
| UniProt Secondary Accession: | O60344,Q6NTG7, Q6UW36, Q8NFD7, Q96NX3, Q96NX4, Q9BRZ8 A5PLK8, |
| UniProt Related Accession: | O60344 |
| Molecular Weight: | 91,211 Da |
| NCBI Full Name: | Endothelin-converting enzyme 2 |
| NCBI Synonym Full Names: | endothelin converting enzyme 2 |
| NCBI Official Symbol: | ECE2 |
| NCBI Protein Information: | endothelin-converting enzyme 2 |
| UniProt Protein Name: | Endothelin-converting enzyme 2 |
| Protein Family: | Endothelin-converting enzyme |
| UniProt Gene Name: | ECE2 |
| UniProt Entry Name: | ECE2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ECE2 antibody (CAB7049) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
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