Cell Death Antibodies 2
Anti-EAF2 Antibody (CAB7119)
- SKU:
- CAB7119
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Description
| Antibody Name: | Anti-EAF2 Antibody |
| Antibody SKU: | CAB7119 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human EAF2 (NP_060926.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, Mouse kidney, Mouse spleen, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human EAF2 (NP_060926.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MNSA AGFS HLDR RERV LKLG ESFE KQPR CAFH TVRY DFKP ASID TSSE GYLE VGEG EQVT ITLP NIEG STPP VTVF KGSK KPYL KECI LIIN HDTG ECRL EKLS SNIT VKKT RVEG SSKI QYRK EQQQ QQMW NSAR TPNL VKHS PSED KMSP ASPI DDIE RELK AEAS LM |
| Gene ID: | 55840 |
| Uniprot: | Q96CJ1 |
| Cellular Location: | Nucleus speckle |
| Calculated MW: | 14kDa/28kDa |
| Observed MW: | 35kDa |
| Synonyms: | EAF2, BM040, TRAITS, U19 |
| Background: |
| UniProt Protein Function: | EAF2: Acts as a transcriptional transactivator of TCEA1 elongation activity. Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivo. Belongs to the EAF family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 3q13.33 Cellular Component: transcription elongation factor complex; nuclear speck Molecular Function:protein binding Biological Process: transcription, DNA-dependent; apoptosis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth |
| UniProt Code: | Q96CJ1 |
| NCBI GenInfo Identifier: | 73919269 |
| NCBI Gene ID: | 55840 |
| NCBI Accession: | Q96CJ1.1 |
| UniProt Related Accession: | Q96CJ1 |
| Molecular Weight: | 35kDa |
| NCBI Full Name: | ELL-associated factor 2 |
| NCBI Synonym Full Names: | ELL associated factor 2 |
| NCBI Official Symbol: | EAF2 |
| NCBI Official Synonym Symbols: | U19; BM040; TRAITS |
| NCBI Protein Information: | ELL-associated factor 2 |
| UniProt Protein Name: | ELL-associated factor 2 |
| UniProt Synonym Protein Names: | Testosterone-regulated apoptosis inducer and tumor suppressor protein |
| Protein Family: | ELL-associated factor |
| UniProt Gene Name: | EAF2 |
| UniProt Entry Name: | EAF2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EAF2 antibody (CAB7119) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunofluorescence analysis of A-549 cells using EAF2 antibody (CAB7119). |
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