Cell Cycle Antibodies 1
Anti-E2F7 Antibody (CAB15211)
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- SKU:
- CAB15211
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-E2F7 Antibody |
| Antibody SKU: | CAB15211 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 540-740 of human E2F7 (NP_976328.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, SP2/0, Mouse spleen, Rat spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 540-740 of human E2F7 (NP_976328.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LLAG QPLV YVPS ASLF MLYG SLQE GPAS GSGS ERDD RSSE APAT VELS SAPS AQKR LCEE RKPQ EEDE PATK RQSR EYED GPLS LVMP KKPS DSTD LASP KTMG NRAS IPLK DIHV NGQL PAAE EISG KATA NSLV SSEW GNPS RNTD VEKP SKEN ESTK EPSL LQYL CVQS PAGL NGFN VLLS GSQT PPTV GPSS GQLP S |
| Gene ID: | 144455 |
| Uniprot: | Q96AV8 |
| Cellular Location: | Nucleus |
| Calculated MW: | 80kDa/98kDa/99kDa |
| Observed MW: | 105kDa |
| Synonyms: | E2F7 |
| Background: |
| UniProt Protein Function: | E2F7: Along with E2F8, inhibitor of E2F-dependent transcription that is important for the control of the E2F1-TP53 apoptotic pathway. Directly represses E2F1 transcription. Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession. Belongs to the E2F/DP family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 12q21.2 Cellular Component: nucleoplasm Molecular Function:identical protein binding; protein binding; transcription corepressor activity; transcription factor activity Biological Process: DNA damage response, signal transduction by p53 class mediator; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; negative regulation of cell proliferation; negative regulation of cytokinesis; negative regulation of transcription from RNA polymerase II promoter; placenta development; positive regulation of DNA endoreduplication; positive regulation of transcription from RNA polymerase II promoter; sprouting angiogenesis |
| NCBI Summary: | E2F transcription factors, such as E2F7, play an essential role in the regulation of cell cycle progression (Di Stefano et al., 2003 [PubMed 14633988]).[supplied by OMIM, May 2008] |
| UniProt Code: | Q96AV8 |
| NCBI GenInfo Identifier: | 311033456 |
| NCBI Gene ID: | 144455 |
| NCBI Accession: | Q96AV8.3 |
| UniProt Secondary Accession: | Q96AV8,A6NC74, B2RMR7, B3KTZ5, B3KUP8, B5MED9, |
| UniProt Related Accession: | Q96AV8 |
| Molecular Weight: | 98,352 Da |
| NCBI Full Name: | Transcription factor E2F7 |
| NCBI Synonym Full Names: | E2F transcription factor 7 |
| NCBI Official Symbol: | E2F7 |
| NCBI Protein Information: | transcription factor E2F7 |
| UniProt Protein Name: | Transcription factor E2F7 |
| Protein Family: | Transcription factor |
| UniProt Gene Name: | E2F7 |
| UniProt Entry Name: | E2F7_HUMAN |
 | Western blot analysis of extracts of various cell lines, using E2F7 antibody (CAB15211) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Western blot analysis of extracts of various cell lines, using E2F7 antibody (CAB15211) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
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