Cell Cycle Antibodies 2

Anti-E2F6 Antibody (CAB11546)

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SKU:
CAB11546
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cell Cycle

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-E2F6 Antibody
Antibody SKU:CAB11546
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human E2F6
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:293T, HepG2, RD
Immunogen:A synthesized peptide derived from human E2F6
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:1876
Uniprot:O75461
Cellular Location:
Calculated MW:32kDa
Observed MW:36KDa
Western blot - E2F6 Rabbit mAb Western blot analysis of extracts of various cell lines, using E2F6 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunoprecipitation - E2F6 Rabbit mAb Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug E2F6 antibody at a dilition of 1:1000.
Chromatin Immunoprecipitation - E2F6 Rabbit mAb Chromatin immunoprecipitation analysis of extracts of 293T cells, using E2F6 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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