Cell Biology Antibodies 6

Anti-E2F5 Antibody (CAB16042)

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SKU:
CAB16042
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-E2F5 Antibody
Antibody SKU:CAB16042
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 197-346 of human E2F5 (NP_001942.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:A-549
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 197-346 of human E2F5 (NP_001942.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QLEV PIPE MGQN GQKK YQIN LKSH SGPI HVLL INKE SSSS KPVV FPVP PPDD LTQP SSQS LTPV TPQK SSMA TQNL PEQH VSER SQAL QQTS ATDI SSAG SISG DIID ELMS SDVF PLLR LSPT PADD YNFN LDDN EGVC DLFD VQIL NY
Gene ID:1875
Uniprot:Q15329
Cellular Location:Nucleus
Calculated MW:20kDa/37kDa
Observed MW:38kDa
Synonyms:E2F5, E2F-5
Background:The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing results in multiple variants encoding different isoforms.
UniProt Protein Function:E2F5: a member of the E2F/DP family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation. Differentially phosphorylated and is expressed in a wide variety of human tissues. It is more homologous to E2F4 than to other E2F/DP family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB.
UniProt Protein Details:

Protein type:Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 8q21.2

Cellular Component: nucleolus; nucleoplasm; nucleus

Molecular Function:protein binding; transcription factor binding

Biological Process: positive regulation of transcription from RNA polymerase II promoter

NCBI Summary:The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing results in multiple variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:Q15329
NCBI GenInfo Identifier:2494230
NCBI Gene ID:1875
NCBI Accession:Q15329.1
UniProt Secondary Accession:Q15329,Q16601, Q92756, E9PBN9,
UniProt Related Accession:Q15329
Molecular Weight:20,192 Da
NCBI Full Name:Transcription factor E2F5
NCBI Synonym Full Names:E2F transcription factor 5
NCBI Official Symbol:E2F5  
NCBI Official Synonym Symbols:E2F-5  
NCBI Protein Information:transcription factor E2F5
UniProt Protein Name:Transcription factor E2F5
Protein Family:Transcription factor
UniProt Gene Name:E2F5  
UniProt Entry Name:E2F5_HUMAN
Anti-E2F5 Antibody (CAB16042)Western blot analysis of extracts of A-549 cells, using E2F5 antibody (CAB16042) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
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