Cell Biology Antibodies 3

Anti-DYNC2H1 Antibody (CAB13126)

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SKU:
CAB13126

Description

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Antibody Name:Anti-DYNC2H1 Antibody
Antibody SKU:CAB13126
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human DYNC2H1 (NP_001073932.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Rat
Positive Samples:Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human DYNC2H1 (NP_001073932.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MANG TADV RKLF IFTT TQNY FGLM SELW DQPL LCNC LEIN NFLD DGNQ MLLR VQRS DAGI SFSN TIEF GDTK DKVL VFFK LRPE VITD ENLH DNIL VSSM LESP ISSL YQAV RQVF APML LKDQ EWSR NFDP KLQN LLSE LEAG LGIV LRRS DTNL TKLK FKED DTRG ILTP SDEF QFWI EQAH RGNK QISK ERAN YFKE LFET IARE FYNL DSLS LLEV VDLV ETTQ DVVD DVWR QTEH DHYP ESRM LH
Gene ID:79659
Uniprot:Q8NCM8
Cellular Location:Cell membrane, Cytoplasm, Peripheral membrane protein, cilium axoneme, cytoskeleton
Calculated MW:105kDa/492kDa/493kDa
Observed MW:492kDa
Synonyms:DYNC2H1, ATD3, DHC1b, DHC2, DNCH2, DYH1B, SRPS2B, SRTD3, hdhc11
Background:This gene encodes a large cytoplasmic dynein protein that is involved in retrograde transport in the cilium and has a role in intraflagellar transport, a process required for ciliary/flagellar assembly. Mutations in this gene cause a heterogeneous spectrum of conditions related to altered primary cilium function and often involve polydactyly, abnormal skeletogenesis, and polycystic kidneys. Alternative splicing results in multiple transcript variants encoding distinct proteins.
UniProt Protein Function:DYNC2H1: May function as a motor for intraflagellar retrograde transport. Functions in cilia biogenesis. May play a role in transport between endoplasmic reticulum and Golgi or organization of the Golgi in cells. Defects in DYNC2H1 are the cause of asphyxiating thoracic dystrophy type 3 (ATD3). ATD3 is an autosomal recessive osteochondrodysplasia which often leads to death in infancy because of a severely constricted thoracic cage and respiratory insufficiency. Defects in DYNC2H1 are the cause of short rib-polydactyly syndrome type 3 (SRPS3); also called Verma-Naumoff syndrome. A lethal skeletal dysplasia characterized by markedly short ribs, short limbs, polydactyly, narrow thorax, and multiple anomalies of major organs, including heart, intestines, genitalia, kidney, liver, and pancreas. Belongs to the dynein heavy chain family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q21-q22.1

Cellular Component: dynein complex; Golgi apparatus; microtubule; apical part of cell; plasma membrane; motile primary cilium; axoneme; cytosol

Molecular Function:ATPase activity; motor activity; microtubule motor activity; ATP binding

Biological Process: asymmetric protein localization; dorsal/ventral pattern formation; organelle organization and biogenesis; forebrain development; positive regulation of smoothened signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class II; cilium biogenesis; protein processing; determination of left/right symmetry; spinal cord motor neuron differentiation; Golgi organization and biogenesis; embryonic limb morphogenesis

Disease: Short-rib Thoracic Dysplasia 3 With Or Without Polydactyly

NCBI Summary:This gene encodes a large cytoplasmic dynein protein that is involved in retrograde transport in the cilium and has a role in intraflagellar transport, a process required for ciliary/flagellar assembly. Mutations in this gene cause a heterogeneous spectrum of conditions related to altered primary cilium function and often involve polydactyly, abnormal skeletogenesis, and polycystic kidneys. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jan 2010]
UniProt Code:Q8NCM8
NCBI GenInfo Identifier:283806679
NCBI Gene ID:79659
NCBI Accession:NP_001368.2
UniProt Secondary Accession:Q8NCM8,O00432, Q16693, Q3C1U8, Q4AC93, Q6ZMX7, Q6ZUM6 Q7Z363, Q8N977, Q92815, Q9HAE4,
UniProt Related Accession:Q8NCM8
Molecular Weight:105,369 Da
NCBI Full Name:cytoplasmic dynein 2 heavy chain 1 isoform 1
NCBI Synonym Full Names:dynein, cytoplasmic 2, heavy chain 1
NCBI Official Symbol:DYNC2H1  
NCBI Official Synonym Symbols:ATD3; DHC2; DHC1b; DNCH2; DYH1B; SRTD3; SRPS2B; hdhc11  
NCBI Protein Information:cytoplasmic dynein 2 heavy chain 1; dynein heavy chain 11; dynein heavy chain isotype 1B; dynein heavy chain, isotype 1B; dynein cytoplasmic heavy chain 2; dynein, cytoplasmic, heavy polypeptide 2
UniProt Protein Name:Cytoplasmic dynein 2 heavy chain 1
UniProt Synonym Protein Names:Cytoplasmic dynein 2 heavy chain; Dynein cytoplasmic heavy chain 2; Dynein heavy chain 11; hDHC11; Dynein heavy chain isotype 1B
UniProt Gene Name:DYNC2H1  
UniProt Entry Name:DYHC2_HUMAN
Anti-DYNC2H1 Antibody (CAB13126)Western blot analysis of extracts of rat testis, using DYNC2H1 antibody (CAB13126) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
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Additional Information

Product Type:
Antibody
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
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