Cell Biology Antibodies 12
Anti-Dynactin subunit 6 Antibody (CAB8316)
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- SKU:
- CAB8316
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | DCTN6 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8316 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human DCTN6 (NP_006562.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | A-549, U-251MG, 22Rv1, Mouse brain, Mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human DCTN6 (NP_006562.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAEK TQKS VKIA PGAV VCVE SEIR GDVT IGPR TVIH PKAR IIAE AGPI VIGE GNLI EEQA LIIN AYPD NITP DTED PEPK PMII GTNN VFEV GCYS QAMK MGDN NVIE SKAY VGRN VILT SGCI IGAC CNLN TFEV IPEN TVIY GADC LRRV QTER PQPQ TLQL DFLM KILP NYHH LKKT MKGS STPV KN |
| Gene ID: | 10671 |
| Uniprot: | O00399 |
| Cellular Location: | Chromosome, Cytoplasm, centromere, cytoskeleton, kinetochore |
| Calculated MW: | 20kDa |
| Observed MW: | 21kDa |
| Synonyms: | DCTN6, WS-3, WS3, p27 |
| Background: | The protein encoded by this gene contains an RGD (Arg-Gly-Asp) motif in the N-terminal region, which confers adhesive properties to macromolecular proteins like fibronectin. It shares a high degree of sequence similarity with the mouse homolog, which has been suggested to play a role in mitochondrial biogenesis. The exact biological function of this gene is not known. |
| UniProt Protein Function: | dynactin 6: contains an RGD (Arg-Gly-Asp) motif in the N-terminal region, which confers adhesive properties to macromolecular proteins like fibronectin. It shares a high degree of sequence similarity with the mouse homolog, which has been suggested to play a role in mitochondrial biogenesis. The exact biological function of this gene is not known. [provided by RefSeq, Jul 2008] |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 8p12 Cellular Component: centrosome; cytosol Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; ER to Golgi vesicle-mediated transport |
| NCBI Summary: | The protein encoded by this gene contains an RGD (Arg-Gly-Asp) motif in the N-terminal region, which confers adhesive properties to macromolecular proteins like fibronectin. It shares a high degree of sequence similarity with the mouse homolog, which has been suggested to play a role in mitochondrial biogenesis. The exact biological function of this gene is not known. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O00399 |
| NCBI GenInfo Identifier: | 3024856 |
| NCBI Gene ID: | 10671 |
| NCBI Accession: | O00399.1 |
| UniProt Secondary Accession: | O00399,B2RAC1, |
| UniProt Related Accession: | O00399 |
| Molecular Weight: | 20,747 Da |
| NCBI Full Name: | Dynactin subunit 6 |
| NCBI Synonym Full Names: | dynactin subunit 6 |
| NCBI Official Symbol: | DCTN6 |
| NCBI Official Synonym Symbols: | WS3; p27; WS-3 |
| NCBI Protein Information: | dynactin subunit 6 |
| UniProt Protein Name: | Dynactin subunit 6 |
| UniProt Synonym Protein Names: | Dynactin subunit p27; Protein WS-3 |
| UniProt Gene Name: | DCTN6 |
| UniProt Entry Name: | DCTN6_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DCTN6 antibody (CAB8316) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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