Antibody Name:	Anti-DVL1 Antibody
Antibody SKU:	CAB10536
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 510-640 of human DVL1 (NP_004412.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 510-640 of human DVL1 (NP_004412.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GQGY PYQY PGPP PCFP PAYQ DPGF SYGS GSTG SQQS EGSK SSGS TRSS RRAP GREK ERRA AGAG GSGS ESDH TAPS GVGS SWRE RPAG QLSR GSSP RSQA SATA PGLP PPHP TTKA YTVV GGPP GGPP VRE
Gene ID:	1855
Uniprot:	O14640
Cellular Location:	Cell membrane, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Peripheral membrane protein, cytosol
Calculated MW:	72kDa/75kDa
Observed MW:	72kDa

Synonyms:

DVL1, DRS2, DVL, DVL1L1, DVL1P1

Background:

DVL1, the human homolog of the Drosophila dishevelled gene (dsh) encodes a cytoplasmic phosphoprotein that regulates cell proliferation, acting as a transducer molecule for developmental processes, including segmentation and neuroblast specification. DVL1 is a candidate gene for neuroblastomatous transformation. The Schwartz-Jampel syndrome and Charcot-Marie-Tooth disease type 2A have been mapped to the same region as DVL1. The phenotypes of these diseases may be consistent with defects which might be expected from aberrant expression of a DVL gene during development.

UniProt Protein Function:	DVL1: Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes. Required for LEF1 activation upon WNT1 and WNT3A signaling. DVL1 and PAK1 form a ternary complex with MUSK which is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ). Belongs to the DSH family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Inhibitor Chromosomal Location of Human Ortholog: 1p36 Cellular Component: cytoplasmic membrane-bound vesicle; cytoplasmic vesicle; cytosol; growth cone; lateral plasma membrane; neuron projection; synapse Molecular Function:enzyme binding; frizzled binding; identical protein binding; protein binding; protein kinase binding Biological Process: dendrite morphogenesis; negative regulation of protein binding; negative regulation of protein kinase activity; neural tube development; neuromuscular junction development; neurotransmitter secretion; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of transcription, DNA-dependent; positive regulation of Wnt receptor signaling pathway; protein stabilization; receptor clustering; regulation of neurotransmitter levels; synapse organization and biogenesis; synaptogenesis; transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; Wnt receptor signaling pathway, planar cell polarity pathway Disease: Robinow Syndrome, Autosomal Dominant; Robinow Syndrome, Autosomal Dominant 2
NCBI Summary:	DVL1, the human homolog of the Drosophila dishevelled gene (dsh) encodes a cytoplasmic phosphoprotein that regulates cell proliferation, acting as a transducer molecule for developmental processes, including segmentation and neuroblast specification. DVL1 is a candidate gene for neuroblastomatous transformation. The Schwartz-Jampel syndrome and Charcot-Marie-Tooth disease type 2A have been mapped to the same region as DVL1. The phenotypes of these diseases may be consistent with defects which might be expected from aberrant expression of a DVL gene during development. [provided by RefSeq, Jul 2008]
UniProt Code:	O14640
NCBI GenInfo Identifier:	145559469
NCBI Gene ID:	1855
NCBI Accession:	O14640.2
UniProt Secondary Accession:	O14640,Q5TA33, Q5TA35,
UniProt Related Accession:	P54792,O14640
Molecular Weight:	72,881 Da
NCBI Full Name:	Segment polarity protein dishevelled homolog DVL-1
NCBI Synonym Full Names:	dishevelled segment polarity protein 1
NCBI Official Symbol:	DVL1
NCBI Official Synonym Symbols:	DVL; DRS2; DVL1L1; DVL1P1
NCBI Protein Information:	segment polarity protein dishevelled homolog DVL-1
UniProt Protein Name:	Segment polarity protein dishevelled homolog DVL-1
UniProt Synonym Protein Names:	DSH homolog 1
Protein Family:	Segment polarity protein dishevelled
UniProt Gene Name:	DVL1
UniProt Entry Name:	DVL1_HUMAN

	Western blot analysis of extracts of Mouse kidney, using DVL1 antibody (CAB10536) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat testis using DVL1 antibody (CAB10536) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using DVL1 antibody (CAB10536) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of NIH/3T3 cells using DVL1 antibody (CAB10536) at dilution of 1:100. Blue: DAPI for nuclear staining.