Antibody Name:	Anti-DUSP6 Antibody
Antibody SKU:	CAB3171
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human DUSP6 (NP_001937.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human DUSP6 (NP_001937.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MIDT LRPV PFAS EMAI SKTV AWLN EQLE LGNE RLLL MDCR PQEL YESS HIES AINV AIPG IMLR RLQK GNLP VRAL FTRG EDRD RFTR RCGT DTVV LYDE SSSD WNEN TGGE SVLG LLLK KLKD EGCR AFYL EGGF SKFQ AEFS LHCE TNLD GSCS SSSP PLPV LGLG GL
Gene ID:	1848
Uniprot:	Q16828
Cellular Location:	Cytoplasm
Calculated MW:	26kDa/42kDa
Observed MW:	42kDa

Synonyms:

DUSP6, HH19, MKP3, PYST1

Background:

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Mutations in this gene have been associated with congenital hypogonadotropic hypogonadism. Alternatively spliced transcript variants have been found for this gene.

UniProt Protein Function:	Inactivates MAP kinases. Has a specificity for the ERK family (PubMed:9858808). Plays an important role in alleviating chronic postoperative pain. Necessary for the normal dephosphorylation of the long-lasting phosphorylated forms of spinal MAPK1/3 and MAP kinase p38 induced by peripheral surgery, which drives the resolution of acute postoperative allodynia.
NCBI Summary:	The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Mutations in this gene have been associated with congenital hypogonadotropic hypogonadism. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2014]
UniProt Code:	Q16828
NCBI GenInfo Identifier:	108860971
NCBI Gene ID:	1848
NCBI Accession:	Q16828.2
UniProt Secondary Accession:	Q16828,O75109, Q53Y75, Q9BSH6,
UniProt Related Accession:	Q16828
Molecular Weight:	26,468 Da
NCBI Full Name:	Dual specificity protein phosphatase 6
NCBI Synonym Full Names:	dual specificity phosphatase 6
NCBI Official Symbol:	DUSP6
NCBI Official Synonym Symbols:	HH19; MKP3; PYST1
NCBI Protein Information:	dual specificity protein phosphatase 6
UniProt Protein Name:	Dual specificity protein phosphatase 6
UniProt Synonym Protein Names:	Dual specificity protein phosphatase PYST1; Mitogen-activated protein kinase phosphatase 3; MAP kinase phosphatase 3; MKP-3
Protein Family:	Dual specificity protein phosphatase
UniProt Gene Name:	DUSP6
UniProt Entry Name:	DUS6_HUMAN

	Western blot analysis of extracts of mouse liver, using DUSP6 antibody (CAB3171) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat brain using DUSP6 Rabbit pAb (CAB3171) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using DUSP6 Rabbit pAb (CAB3171) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.