Name: Anti-DUSP5 Antibody (CAB10212)
Brand: Cell Biology Antibodies 1
SKU: CAB10212
Availability: OutOfStock

Antibody Name:	Anti-DUSP5 Antibody
Antibody SKU:	CAB10212
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 315-384 of human DUSP5 (NP_004410.3).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	Raji

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 315-384 of human DUSP5 (NP_004410.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SEIL PSTP NPQP PSCQ GEAA GSSL IGHL QTLS PDMQ GAYC TFPA SVLA PVPT HSTV SELS RSPV ATAT SC
Gene ID:	1847
Uniprot:	Q16690
Cellular Location:	Nucleus
Calculated MW:	42kDa
Observed MW:	42kDa

Synonyms:

DUSP5, DUSP, HVH3

Background:

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, is expressed in a variety of tissues with the highest levels in pancreas and brain, and is localized in the nucleus.

UniProt Protein Function:	Function: Displays phosphatase activity toward several substrates. The highest relative activity is toward ERK1.
UniProt Protein Details:	Catalytic activity: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.A phosphoprotein + H2O = a protein + phosphate. Subcellular location: Nucleus Potential. Sequence similarities: Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.Contains 1 rhodanese domain.Contains 1 tyrosine-protein phosphatase domain.
NCBI Summary:	The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, is expressed in a variety of tissues with the highest levels in pancreas and brain, and is localized in the nucleus. [provided by RefSeq, Jul 2008]
UniProt Code:	Q16690
NCBI GenInfo Identifier:	62865890
NCBI Gene ID:	1847
NCBI Accession:	NP_004410
UniProt Related Accession:	Q16690
Molecular Weight:	42kDa
NCBI Full Name:	dual specificity protein phosphatase 5
NCBI Synonym Full Names:	dual specificity phosphatase 5
NCBI Official Symbol:	DUSP5
NCBI Official Synonym Symbols:	DUSP; HVH3
NCBI Protein Information:	dual specificity protein phosphatase 5
UniProt Protein Name:	Dual specificity protein phosphatase 5
UniProt Synonym Protein Names:	Dual specificity protein phosphatase hVH3
Protein Family:	Dual specificity protein phosphatase
UniProt Gene Name:	DUSP5
UniProt Entry Name:	DUS5_HUMAN

Western blot analysis of extracts of Raji cells, using DUSP5 antibody (CAB10212) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

ELISA Kits

Human DUSP5 / VH3 ELISA Kit

Human Dual specificity protein phosphatase 5 (DUSP5) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)