Cell Biology Antibodies 8
Anti-DUSP4 Antibody (CAB2726)
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- SKU:
- CAB2726
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-DUSP4 Antibody |
| Antibody SKU: | CAB2726 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-168 of human DUSP4 (NP_001385.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human |
| Positive Samples: | COS-7, HeLa, A375 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-168 of human DUSP4 (NP_001385.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MVTM EELR EMDC SVLK RLMN RDEN GGGA GGSG SHGT LGLP SGGK CLLL DCRP FLAH SAGY ILGS VNVR CNTI VRRR AKGS VSLE QILP AEEE VRAR LRSG LYSA VIVY DERS PRAE SLRE DSTV SLVV QALR RNAE RTDI CLLK GGYE RFSS EYPE FCSK TKAL AAIP |
| Gene ID: | 1846 |
| Uniprot: | Q13115 |
| Cellular Location: | Nucleus |
| Calculated MW: | 32kDa/42kDa |
| Observed MW: | 47kDa |
| Synonyms: | DUSP4, HVH2, MKP-2, MKP2, TYP |
| Background: | The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Two alternatively spliced transcript variants, encoding distinct isoforms, have been observed for this gene. In addition, multiple polyadenylation sites have been reported. |
| UniProt Protein Function: | MKP-2: a non-receptor, dual-specificity phosphoprotein phosphatase (DUSP). Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP4 inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Induced by hepatocyte growth factor (HGF) and vascular endothelial cell growth factor (VEGF) in human endothelial cells. Two alternatively spliced isoforms have been described. In addition, multiple polyadenylation sites have been reported. Contains 1 rhodanese domain. |
| UniProt Protein Details: | Protein type:Protein phosphatase, dual-specificity; EC 3.1.3.48; EC 3.1.3.16 Chromosomal Location of Human Ortholog: 8p12-p11 Cellular Component: nucleoplasm; nucleus Molecular Function:MAP kinase tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity; protein tyrosine/threonine phosphatase activity Biological Process: endoderm formation; inactivation of MAPK activity; innate immune response; MAPKKK cascade; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; nerve growth factor receptor signaling pathway; protein amino acid dephosphorylation; stress-activated MAPK cascade; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway |
| NCBI Summary: | The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Two alternatively spliced transcript variants, encoding distinct isoforms, have been observed for this gene. In addition, multiple polyadenylation sites have been reported. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13115 |
| NCBI GenInfo Identifier: | 2499745 |
| NCBI Gene ID: | 1846 |
| NCBI Accession: | Q13115.1 |
| UniProt Secondary Accession: | Q13115,Q13524, B2RBU5, D3DSU4, G5E930, |
| UniProt Related Accession: | Q13115 |
| Molecular Weight: | 32,993 Da |
| NCBI Full Name: | Dual specificity protein phosphatase 4 |
| NCBI Synonym Full Names: | dual specificity phosphatase 4 |
| NCBI Official Symbol: | DUSP4 |
| NCBI Official Synonym Symbols: | TYP; HVH2; MKP2; MKP-2 |
| NCBI Protein Information: | dual specificity protein phosphatase 4 |
| UniProt Protein Name: | Dual specificity protein phosphatase 4 |
| UniProt Synonym Protein Names: | Dual specificity protein phosphatase hVH2; Mitogen-activated protein kinase phosphatase 2; MAP kinase phosphatase 2; MKP-2 |
| Protein Family: | Dual specificity protein phosphatase |
| UniProt Gene Name: | DUSP4 |
| UniProt Entry Name: | DUS4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DUSP4 antibody (CAB2726) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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