Name: Anti-DUSP16 Antibody (CAB10155)
Brand: Cell Biology Antibodies 1
SKU: CAB10155
Availability: OutOfStock

Antibody Name:	Anti-DUSP16 Antibody
Antibody SKU:	CAB10155
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 416-665 of human DUSP16 (NP_085143.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, A-549, K-562, HepG2, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 416-665 of human DUSP16 (NP_085143.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SLHG FSSS EDAL EYYK PSTT LDGT NKLC QFSP VQEL SEQT PETS PDKE EASI PKKL QTAR PSDS QSKR LHSV RTSS SGTA QRSL LSPL HRSG SVED NYHT SFLF GLST SQQH LTKS AGLG LKGW HSDI LAPQ TSTP SLTS SWYF ATES SHFY SASA IYGG SASY SAYS CSQL PTCG DQVY SVRR RQKP SDRA DSRR SWHE ESPF EKQF KRRS CQME FGES IMSE NRSR EELG KVGS QSSF SGSM EIIE VS
Gene ID:	80824
Uniprot:	Q9BY84
Cellular Location:	Cytoplasm, Cytoplasmic vesicle, Nucleus
Calculated MW:	16kDa/73kDa
Observed MW:	73kDa

Synonyms:

DUSP16, MKP-7, MKP7

Background:

This gene encodes a mitogen-activated protein kinase phosphatase that is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. The encoded protein specifically regulates the c-Jun amino-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) pathways.

UniProt Protein Function:	MKP-7: a non-receptor, dual-specificity phosphoprotein phosphatase (DUSP). Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP16 suppresses MAPK activation in the order of selectivity, JNK >> p38 > ERK, but interacts with ERK as well as JNK and p38. Overexpression of DUSP16 in BCR-ABL-transformed fibroblasts reduces their transforming capacity in vitro and in vivo via down regulation of BCR-ABL-induced JNK activation. DUSP16 might be haploinsufficient for tumor suppression. Contains 1 rhodanese domain.
UniProt Protein Details:	Protein type:EC 3.1.3.48; Protein phosphatase, dual-specificity; EC 3.1.3.16 Chromosomal Location of Human Ortholog: 12p13 Cellular Component: nucleoplasm; cytoplasm; cytoplasmic membrane-bound vesicle; nucleus; cytosol Molecular Function:protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity; MAP kinase tyrosine/serine/threonine phosphatase activity; phosphoprotein phosphatase activity Biological Process: MAPK export from nucleus; dephosphorylation; MAPK phosphatase export from nucleus, leptomycin B sensitive; protein amino acid dephosphorylation; inactivation of MAPK activity
NCBI Summary:	This gene encodes a mitogen-activated protein kinase phosphatase that is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. The encoded protein specifically regulates the c-Jun amino-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) pathways.[provided by RefSeq, May 2010]
UniProt Code:	Q9BY84
NCBI GenInfo Identifier:	38372911
NCBI Gene ID:	80824
NCBI Accession:	NP_085143.1
UniProt Secondary Accession:	Q9BY84,Q547C7, Q9C0G3,
UniProt Related Accession:	Q9BY84
Molecular Weight:	73,102 Da
NCBI Full Name:	dual specificity protein phosphatase 16
NCBI Synonym Full Names:	dual specificity phosphatase 16
NCBI Official Symbol:	DUSP16
NCBI Official Synonym Symbols:	MKP7; MKP-7
NCBI Protein Information:	dual specificity protein phosphatase 16; MAPK phosphatase-7; MAP kinase phosphatase 7; mitogen-activated protein kinase phosphatase 7
UniProt Protein Name:	Dual specificity protein phosphatase 16
UniProt Synonym Protein Names:	Mitogen-activated protein kinase phosphatase 7
UniProt Gene Name:	DUSP16
UniProt Entry Name:	DUS16_HUMAN

Western blot analysis of extracts of various cell lines, using DUSP16 antibody (CAB10155) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

DUSP16 Antibody (PACO05405)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)