Cell Biology Antibodies 12

Anti-DUSP10 Antibody (CAB8748)

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SKU:
CAB8748
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-DUSP10 Antibody
Antibody SKU:CAB8748
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DUSP10 (NP_009138.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:BT-474
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DUSP10 (NP_009138.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPPS PLDD RVVV ALSR PVRP QDLN LCLD SSYL GSAN PGSN SHPP VIAT TVVS LKAA NLTY MPSS SGSA RSLN CGCS SASC CTVA TYDK DNQA QTQA IAAG TTTT AIGT STTC PANQ MVNN NENT GSLS PSSG VGSP VSGT PKQL ASIK IIYP NDLA KKMT KCSK SHLP SQGP VIID CRPF MEYN KSHI QGAV HINC ADKI SRRR LQQG KITV LDLI SCRE GKDS FKRI FSKE IIVY DENT NEPS RVMP SQPL HIVL ESLK REGK EPLV LKGG LSSF KQNH ENLC DNSL QLQE CREV GGGA
Gene ID:11221
Uniprot:Q9Y6W6
Cellular Location:Cytoplasm, Nucleus
Calculated MW:16kDa/52kDa
Observed MW:53kDa
Synonyms:DUSP10, MKP-5, MKP5
Background:Dual specificity protein phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAP kinase superfamily, which is associated with cellular proliferation and differentiation. Different members of this family of dual specificity phosphatases show distinct substrate specificities for MAP kinases, different tissue distribution and subcellular localization, and different modes of expression induction by extracellular stimuli. This gene product binds to and inactivates p38 and SAPK/JNK. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:MKP-5: a non-receptor, dual-specificity phosphoprotein phosphatase (DUSP). Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP10 binds to and inactivates p38 and SAPK/JNK, but not MAPK/ERK. Its subcellular localization is unique; it is evenly distributed in both the cytoplasm and the nucleus. This gene is widely expressed in various tissues and organs, and its expression is elevated by stress stimuli. Three transcript variants encoding two different isoforms have been found for this gene. Contains 1 rhodanese domain.
UniProt Protein Details:

Protein type:Protein phosphatase, dual-specificity; Motility/polarity/chemotaxis; EC 3.1.3.48; EC 3.1.3.16

Chromosomal Location of Human Ortholog: 1q41

Cellular Component: cytoplasm; Golgi apparatus; nucleoplasm; nucleus

Molecular Function:MAP kinase phosphatase activity; phosphoprotein phosphatase activity; phosphoric monoester hydrolase activity

Biological Process: inactivation of MAPK activity; JNK cascade; negative regulation of JNK activity; protein amino acid dephosphorylation; response to stress

NCBI Summary:Dual specificity protein phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAP kinase superfamily, which is associated with cellular proliferation and differentiation. Different members of this family of dual specificity phosphatases show distinct substrate specificities for MAP kinases, different tissue distribution and subcellular localization, and different modes of expression induction by extracellular stimuli. This gene product binds to and inactivates p38 and SAPK/JNK. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
UniProt Code:Q9Y6W6
NCBI GenInfo Identifier:20138090
NCBI Gene ID:11221
NCBI Accession:Q9Y6W6.1
UniProt Secondary Accession:Q9Y6W6,Q6GSI4, Q9H9Z5, D3DTB4,
UniProt Related Accession:Q9Y6W6
Molecular Weight:16,110 Da
NCBI Full Name:Dual specificity protein phosphatase 10
NCBI Synonym Full Names:dual specificity phosphatase 10
NCBI Official Symbol:DUSP10  
NCBI Official Synonym Symbols:MKP5; MKP-5  
NCBI Protein Information:dual specificity protein phosphatase 10
UniProt Protein Name:Dual specificity protein phosphatase 10
UniProt Synonym Protein Names:Mitogen-activated protein kinase phosphatase 5; MAP kinase phosphatase 5; MKP-5
Protein Family:Dual specificity protein phosphatase
UniProt Gene Name:DUSP10  
UniProt Entry Name:DUS10_HUMAN
Anti-DUSP10 Polyclonal Antibody (CAB8748)Western blot analysis of extracts of BT-474 cells, using DUSP10 antibody (CAB8748) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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