Epigenetics & Nuclear Signaling Antibodies 4
Anti-DTYMK Antibody (CAB6370)
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- SKU:
- CAB6370
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-DTYMK Antibody |
| Antibody SKU: | CAB6370 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-212 of human DTYMK (NP_036277.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 22Rv1, HeLa, HepG2, Raji, MCF7, Mouse bone marrow, Mouse spleen, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-212 of human DTYMK (NP_036277.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAR RGAL IVLE GVDR AGKS TQSR KLVE ALCA AGHR AELL RFPE RSTE IGKL LSSY LQKK SDVE DHSV HLLF SANR WEQV PLIK EKLS QGVT LVVD RYAF SGVA FTGA KENF SLDW CKQP DVGL PKPD LVLF LQLQ LADA AKRG AFGH ERYE NGAF QERA LRCF HQLM KDTT LNWK MVDA SKSI EAVH EDIR VLSE DAIR TATE KPLG ELWK |
| Gene ID: | 1841 |
| Uniprot: | P23919 |
| Cellular Location: | |
| Calculated MW: | 21kDa/23kDa |
| Observed MW: | 24kDa |
| Synonyms: | DTYMK, CDC8, PP3731, TMPK, TYMK |
| Background: |
| UniProt Protein Function: | DTYMK: Catalyzes the conversion of dTMP to dTDP. Belongs to the thymidylate kinase family. |
| UniProt Protein Details: | Protein type:EC 2.7.4.9; Mitochondrial; Kinase, other; Nucleotide Metabolism - pyrimidine Chromosomal Location of Human Ortholog: 2q37.3 Cellular Component: cytosol; mitochondrion; nucleus Molecular Function:nucleoside phosphate kinase activity; thymidylate kinase activity; uridylate kinase activity Biological Process: cell cycle; cell proliferation; dTDP biosynthetic process; dTTP biosynthetic process; dUDP biosynthetic process; nucleobase, nucleoside and nucleotide interconversion |
| UniProt Code: | P23919 |
| NCBI GenInfo Identifier: | 56405325 |
| NCBI Gene ID: | 1841 |
| NCBI Accession: | P23919.4 |
| UniProt Secondary Accession: | P23919,Q6FGX1, Q9BUX4, B7ZW70, |
| UniProt Related Accession: | P23919 |
| Molecular Weight: | 21,064 Da |
| NCBI Full Name: | Thymidylate kinase |
| NCBI Synonym Full Names: | deoxythymidylate kinase |
| NCBI Official Symbol: | DTYMK |
| NCBI Official Synonym Symbols: | CDC8; TMPK; TYMK; PP3731 |
| NCBI Protein Information: | thymidylate kinase |
| UniProt Protein Name: | Thymidylate kinase |
| UniProt Synonym Protein Names: | dTMP kinase |
| UniProt Gene Name: | DTYMK |
| UniProt Entry Name: | KTHY_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DTYMK antibody (CAB6370) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of L929 cells using DTYMK Rabbit pAb (CAB6370) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using DTYMK Rabbit pAb (CAB6370) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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