Signal Transduction Antibodies 3
Anti-DTX2 Antibody (CAB7398)
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- SKU:
- CAB7398
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-DTX2 Antibody |
| Antibody SKU: | CAB7398 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human DTX2 (NP_065943.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, HepG2, Mouse testis, Mouse spleen, Mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human DTX2 (NP_065943.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAMA PSPS LVQV YTSP AAVA VWEW QDGL GTWH PYSA TVCS FIEQ QFVQ QKGQ RFGL GSLA HSIP LGQA DPSL APYI IDLP SWTQ FRQD TGTM RAVR RHLF PQHS APGR GVVW EWLS DDGS WTAY EASV CDYL EQQV ARGN QLVD LAPL GYNY TVNY TTHT QTNK TSSF CRSV RRQA GPPY PVTT IIAP PGHT GVAC SCHQ CLSG SRTG PVSG RYRH SMTN LPAY PVPQ HPPH RTAS VFGT HQAF APYN KPSL SGAR SAPR LNTT NAWG AAPP SLGS QPLY |
| Gene ID: | 113878 |
| Uniprot: | Q86UW9 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 62kDa/67kDa |
| Observed MW: | 67kDa |
| Synonyms: | DTX2, RNF58 |
| Background: |
| UniProt Protein Function: | DTX2: Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Functions as an ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity. Homodimer. May form a heterodimer with other members of the Deltex family. Interacts with NOTCH1. Belongs to the Deltex family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ubiquitin ligase; Ligase; Ubiquitin conjugating system; EC 6.3.2.-; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 7q11.23 Cellular Component: nucleoplasm; nuclear membrane; cytoplasm Molecular Function:protein binding; zinc ion binding; ligase activity Biological Process: Notch signaling pathway; protein ubiquitination |
| NCBI Summary: | DTX2 functions as an E3 ubiquitin ligase (Takeyama et al., 2003 [PubMed 12670957]).[supplied by OMIM, Nov 2009] |
| UniProt Code: | Q86UW9 |
| NCBI GenInfo Identifier: | 68067880 |
| NCBI Gene ID: | 113878 |
| NCBI Accession: | Q86UW9.3 |
| UniProt Related Accession: | Q86UW9 |
| Molecular Weight: | Observed: 67 kDaPredicted: 68 kDa |
| NCBI Full Name: | Probable E3 ubiquitin-protein ligase DTX2 |
| NCBI Synonym Full Names: | deltex E3 ubiquitin ligase 2 |
| NCBI Official Symbol: | DTX2 |
| NCBI Official Synonym Symbols: | RNF58 |
| NCBI Protein Information: | probable E3 ubiquitin-protein ligase DTX2 |
| UniProt Protein Name: | Probable E3 ubiquitin-protein ligase DTX2 |
| UniProt Synonym Protein Names: | Protein deltex-2; Deltex2; hDTX2; RING finger protein 58 |
| Protein Family: | Protein DETOXIFICATION |
| UniProt Gene Name: | DTX2 |
| UniProt Entry Name: | DTX2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DTX2 antibody (CAB7398) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat liver using DTX2 antibody (CAB7398) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat spleen using DTX2 antibody (CAB7398) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human kidney using DTX2 antibody (CAB7398) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse liver using DTX2 antibody (CAB7398) at dilution of 1:100 (40x lens). |
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