Cell Biology Antibodies 3

Anti-DSP Antibody (CAB13299)

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SKU:
CAB13299
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-DSP Antibody
Antibody SKU:CAB13299
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 2750-2850 of human DSP (NP_004406.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:HepG2
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 2750-2850 of human DSP (NP_004406.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AIRK GFID GRAA QRLQ DTSS YAKI LTCP KTKL KISY KDAI NRSM VEDI TGLR LLEA ASVS SKGL PSPY NMSS APGS RSGS RSGS RSGS RSGS RSGS RRGS F
Gene ID:1832
Uniprot:P15924
Cellular Location:Cell junction, Cytoplasm, cytoskeleton, desmosome
Calculated MW:260kDa/278kDa/331kDa
Observed MW:315kDa
Synonyms:DCWHKTA, DP, DSP
Background:This gene encodes a protein that anchors intermediate filaments to desmosomal plaques and forms an obligate component of functional desmosomes. Mutations in this gene are the cause of several cardiomyopathies and keratodermas, including skin fragility-woolly hair syndrome. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:Desmoplakin: Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin- plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes. Homodimer. Interacts with COL17A1 (via cytoplasmic region). Associates (via C-terminal) with KRT5-KRT14 (via rod region), KRT8-KRT18 and VIM intermediate filaments. Interacts with DSC2. Isoform DPI is apparently an obligate constituent of all desmosomes. Isoform DPII resides predominantly in tissues and cells of stratified origin. Belongs to the plakin or cytolinker family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Cytoskeletal; Cell adhesion

Chromosomal Location of Human Ortholog: 6p24

Cellular Component: desmosome; mitochondrion; basolateral plasma membrane; plasma membrane; intermediate filament; cornified envelope; fascia adherens; nucleus

Molecular Function:protein binding, bridging; protein binding; protein kinase C binding; structural constituent of cytoskeleton; cell adhesion molecule binding; structural molecule activity

Biological Process: keratinocyte differentiation; cell-cell adhesion; epidermis development; wound healing; apoptosis; intermediate filament organization; peptide cross-linking; cell structure disassembly during apoptosis

Disease: Cardiomyopathy, Dilated, With Woolly Hair And Keratoderma; Arrhythmogenic Right Ventricular Dysplasia, Familial, 8; Keratosis Palmoplantaris Striata Ii; Cardiomyopathy, Dilated, With Woolly Hair, Keratoderma, And Tooth Agenesis; Skin Fragility-woolly Hair Syndrome; Epidermolysis Bullosa, Lethal Acantholytic

NCBI Summary:Desmosomes are intercellular junctions that tightly link adjacent cells. Desmoplakin is an obligate component of functional desmosomes that anchors intermediate filaments to desmosomal plaques. The N-terminus of desmoplakin is required for localization to the desmosome and interacts with the N-terminal region of plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds with intermediate filaments. In the mid-region of desmoplakin, a coiled-coiled rod domain is responsible for homodimerization. Mutations in this gene are the cause of several cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus. [provided by RefSeq, Jul 2008]
UniProt Code:P15924
NCBI GenInfo Identifier:58530840
NCBI Gene ID:1832
NCBI Accession:NP_004406.2
UniProt Secondary Accession:P15924,O75993, Q14189, Q9UHN4, B2RTT2, D7RX09,
UniProt Related Accession:P15924
Molecular Weight:278,916 Da
NCBI Full Name:desmoplakin isoform I
NCBI Synonym Full Names:desmoplakin
NCBI Official Symbol:DSP  
NCBI Official Synonym Symbols:DP; DPI; DPII; DCWHKTA  
NCBI Protein Information:desmoplakin
UniProt Protein Name:Desmoplakin
UniProt Synonym Protein Names:250/210 kDa paraneoplastic pemphigus antigen
Protein Family:Desmoplakin
UniProt Gene Name:DSP  
UniProt Entry Name:DESP_HUMAN
Anti-DSP Antibody (CAB13299)Western blot analysis of extracts of HepG2 cells, using DSP antibody (CAB13299) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
Anti-DSP Antibody (CAB13299)Immunohistochemistry of paraffin-embedded human gastric cancer using DSP antibody (CAB13299) at dilution of 1:100 (40x lens).
Anti-DSP Antibody (CAB13299)Immunohistochemistry of paraffin-embedded mouse heart using DSP antibody (CAB13299) at dilution of 1:100 (40x lens).
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